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Table 5 Unigene clusters candidate to encode for miRNAs

From: A computational-based update on microRNAs and their targets in barley (Hordeum vulgare L.)

 

Features of the precursors identified

Best scoring alignment with miRBase precursors

Unigene cluster

ΔG (kcal/mol)

MFEI

NM

ML

PL

Arm

Accession number

miRNA

Score

e-value

Hv.1306

-72.8

0.89

2

21

167

3'

MI0006178

tae-MIR444

736

1 × 10-54

Hv.5064

-22.0

1.00

2

18

68

3'

MI0006199

tae-MIR1137

168

4 × 10-8

Hv.7117

-74.9

0.96

3

21

115

5'

MI0011565

bdi-MIR397

196

5 × 10-10

Hv.8158

-60.7

0.88

3

21

92

5'

MI0001763

sof-MIR168a

393

1 × 10-26

Hv.14657

-31.5

1.85

2

21

69

3'

MI0006183

tae-MIR1121

241

3 × 10-14

Hv.15131

-51.1

0.90

2

21

129

3'

MI0006976

osa-MIR444d

431

2 × 10-29

Hv.16635

-91.7

0.92

3

21

200

3'

MI0006170

tae-MIR159a

805

2 × 10-60

Hv.22601

-34.0

0.92

4

22

97

3'

MI0006192

tae-MIR1130

179

9 × 10-9

Hv.28058

-63.8

1.60

2

24

129

3'

MI0006182

tae-MIR1120

147

7 × 10-6

Hv.29065

-53.6

1.07

4

22

131

5'

MI0006199

tae-MIR1137

182

8 × 10-9

Hv.29519

-42.9

1.02

2

21

96

3'

MI0006192

tae-MIR1130

144

8 × 10-6

Hv.30469

-39.0

0.91

3

21

117

5'

MI0006199

tae-MIR1137

141

2 × 10-5

  1. For each cluster, the table shows details about the putative precursors: the free energy ΔG, the minimal folding free energy index (MFEI), the number of mismatches in miRNA/miRNA* duplex (NM), the mature length (ML), the precursor length (PL) and the location of mature miRNA (3' or 5'). Moreover, it is also reported the more similar known precursor in miRBase, with the alignment score and p-value.