Figure 5

SNP discovery and gene expression profiling in the apicoplast. The T. gondii plastid (apicoplast; RH strain sequence) was tiled at a 25 nt resolution on alternating strands allowing probe level expression profiling across the entire organelle. Expression patterns (inner circle; red and blue bars represent opposite strands and high absolute expression; grey bars represent low expression levels) are consistent with an operon transcription, with two major origins of transcription evident at the LSU rRNA genes, running in opposite directions (as indicated by the arrows). SFPs were also uncovered using DNA hybridization differences between GT1 (type I), Pru (type II), and CTG (type III), revealing 43 type II SNPs (green diamonds), 12 type III SNPs (blue diamonds), and no type I SNPs (red diamonds).