Skip to main content
Figure 2 | BMC Genomics

Figure 2

From: Structural modelling and comparative analysis of homologous, analogous and specific proteins from Trypanosoma cruzi versus Homo sapiens: putative drug targets for chagas' disease treatment

Figure 2

Structural and sequence comparison between 2,4-dienoyl CoA reductase (DECR) from Trypanosoma cruzi and Homo sapiens , analogous enzymes. 2(a): sequence alignment between putative paralogous DECR enzymes from T. cruzi and mitochondrial DECR1 and peroxisomal DECR2 enzymes from H. sapiens. The alignment was performed using ClustalX (v1.83) [46]. 2(b): structural alignment between the H. sapiens DECR1 (reconstructed PDB: 1W6U) (yellow) and DECR2 model (blue), constructed using PDB: 1W6U as template. The active site residues Y199 and K214 of DECR1 (yellow) are presented in detail, according to [47], and L167 and K182 of DECR2 (blue), which were inferred by the structural alignment with DECR1. 2(c): structural alignment between DECR enzymes of T. cruzi. The putative active sites constituted by Y175 and H261 of Tc00.1047053509941.100 (yellow) and, G180 and H269 of Tc00.1047053510303.210 (blue) are presented in detail. The active sites of T. cruzi DECR were inferred by their structural alignment (not presented) with the DECR protein (PDB: 1PS9) from Escherichia coli, used as template. Its active site residues Y166 and H252 are described by [36]. The alignments were performed by Swiss-PDB Viewer (v4.0.1) program [31] and the images were generated using VMD (Visual Molecular Dynamics - v1.8.6) software [45]

Back to article page