Figure 6

Functional network predicted from the genes expressed differentially during Cr (VI) stress in rice (For detail pathway please visit http://www.nbri.res.in/chromiumpathway/Cr_final.html). The rice variety IR-64 was germinated and allowed to grow for 5 d at 37°C and then transferred to Hewitt solution for growth. After 10 d of growth, seedlings of uniform size and growth were treated with 100 μM of Cr (VI) under standard physiological conditions. The microarray was performed using one-cycle target labeling and control reagents (Affymetrix, USA) using 5 μ g RNA The hybridization data were analyzed using dCHIP software. The differentially expressed transcripts ranked by the magnitude of the moderated t-statistic determined from the statistical analysis were analyzed for cellular pathway and transcriptome interactions using the program Pathway Studio (Ariadne Genomics, USA). The pathway diagram was further filtered to show the proteins involved in cellular processes associated with the Cr response network.