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Table 5 Candidate genes for the improvement of malting quality.

From: Transcriptome analysis of a barley breeding program examines gene expression diversity and reveals target genes for malting quality improvement

Probe Set Name

E value

Putative function

ME

GP

S/T

DP

AA

BG

Map loc.

"Out of steep"

         

Contig10584_at

_

No hit

**(+)

      

Contig12460_at

7.00E-22

Hypothetical protein P0657H12.23 (Oryza sativa)

 

**(-)

   

*(+)

 

Contig13150_at

_

No hit

  

*(+)

    

Contig14046_at

1.00E-21

Hypothetical protein OJ1756_H07 (Oryza sativa)

  

*(-)

 

*(-)

  

Contig17218_at

_

No hit

**(+)

   

*(+)

  

Contig17647_at

2.00E-29

Os08g0100400 protein

*(-)

   

*(-)

  

Contig21675_at

_

No hit

***(+)

   

**(+)

*(-)

 

Contig22323_at

6.00E-38

26S protease regulatory subunit 6B

**(+)

   

*(+)

  

Contig23156_at

_

No hit

 

*(-)

 

*(-)

 

**(+)

 

Contig25703_at

2.00E-32

F-box domain containing protein

**(+)

    

*(-)

 

Contig2885_at

4.00E-60

Eukaryotic translation initiation factor 5 (Zea mays)

 

*(+)

   

**(-)

 

Contig3129_at

1.00E-124

Mitochondrial prohibitin complex protein 2 (Oryza sativa)

*(-)

 

**(-)

 

*(-)

  

Contig3173_at

2.00E-23

Low temperature and salt responsive protein (Pennisetum americanum)

     

*(+)

2H (51.75)

Contig3191_at

1.00E-121

Mitochondrial ATP synthase (Triticum aestivum)

    

*(+)

  

Contig3339_at

1.00E-115

Protein CP5

 

*(+)

   

*(-)

 

Contig3668_at

1.00E-115

Ice recrystallization inhibition protein 1 precursor (Triticum aestivum)

  

*(-)

    

Contig3861_at

_

No hit

*(-)

*(-)

*(-)

 

***(-)

  

Contig3863_s_at

_

No hit

 

*(-)

*(-)

 

**(-)

  

Contig398_s_at

6.00E-91

6,7-dimethyl-8-ribityllumazine synthase, chloroplast precursor

     

*(-)

 

Contig4837_at

1.00E-52

Small nuclear ribonucleoprotein Sm D2

  

*(+)

 

**(+)

  

Contig4839_at

5.00E-52

Small nuclear ribonucleoprotein Sm D2

    

*(-)

  

Contig6845_at

_

No hit

*(-)

   

*(-)

  

Contig9672_at

7.00E-52

Unknown

 

*(+)

*(+)

 

**(+)

 

3H (162.15)

Contig9733_at

9.00E-98

RING-H2 finger protein

*(-)

      

EBem10_SQ004_D16_at

_

No hit

   

*(+)

 

*(-)

 

EBpi01_SQ001_C06_at

_

No hit

***(-)

   

*(-)

  

HB20B24r_s_at

2.00E-85

Hordoindoline b (Hordeum vulgare)

*(+)

      

HS04A09u_s_at

_

No hit

***(+)

   

*(+)

 

7H (140.21)

HT12D12u_s_at

5.00E-48

Glutaredoxin (Triticum aestivum)

   

*(-)

   

HV_CEa0001D21r2_at

_

No hit

 

**(+)

   

*(-)

2HS

HV_CEa0011J09r2_at

2.00E-18

Putative B12D protein (Oryza sativa)

**(-)

    

**(+)

1H (128.14)

HVSMEa0001B10r2_x_at

_

No hit

    

*(-)

  

HVSMEl0003B06r2_at

_

No hit

**(+)

   

*(+)

  

HVSMEl0007F21r2_s_at

_

No hit

**(+)

      

HVSMEl0023H09r2_at

_

No hit

     

*(-)

 

rbags14a14_at

2.00E-36

Putative prolyl endopeptidase (Oryza sativa)

 

*(-)

     

rbags14a14_s_at

2.00E-36

Putative prolyl endopeptidase (Oryza sativa)

 

**(-)

    

6H (45.44)\

"3d of germination"

        

Contig12150_at

_

No hit

 

*(-)

 

*(-)

  

7H (140.21)

Contig12460_at

7.00E-22

Hypothetical protein P0657H12.23 (Oryza sativa)

 

**(-)

 

*(-)

 

*(+)

 

Contig13847_s_at

3.00E-85

Putative subtilisin-like serine protease (Oryza sativa)

**(+)

    

*(-)

 

Contig14046_at

1.00E-21

Hypothetical protein OJ1756_H07 (Oryza sativa)

  

**(-)

 

*(-)

  

Contig14709_at

3.00E-37

Os01g0579800 protein

**(-)

 

*(-)

 

*(-)

 

1H (138.31)

Contig159_at

3.00E-53

Histone H4

*(+)

*(+)

*(+)

 

***(+)

  

Contig16647_at

6.00E-42

Os12g0566100 protein

  

**(-)

 

*(-)

  

Contig17218_at

_

No hit

**(+)

   

*(+)

  

Contig173_at

6.00E-54

Histone H4

    

*(-)

  

Contig17647_at

2.00E-29

Os08g0100400 protein

**(-)

   

*(-)

  

Contig22323_at

6.00E-38

26S protease regulatory subunit 6B

**(+)

   

*(+)

  

Contig2885_at

4.00E-60

Eukaryotic translation initiation factor 5 (Zea mays)

 

*(+)

 

*(+)

 

**(-)

 

Contig3129_at

1.00E-124

Mitochondrial prohibitin complex protein 2 (Oryza sativa)

  

**(-)

 

*(-)

  

Contig3668_at

1.00E-115

Ice recrystallization inhibition protein 1 precursor (Triticum aestivum)

  

*(-)

    

Contig3863_s_at

_

No hit

 

*(-)

*(-)

 

**(-)

  

Contig4384_at

1.00E-92

DAG protein, chloroplast precursor

    

*(-)

 

5H (103.92)

Contig500_at

2.00E-53

Histone H4

 

*(-)

*(-)

 

**(-)

  

Contig5185_at

1.00E-145

RNase S-like protein (Hordeum vulgare)

*(-)

     

1H (135.56)

Contig6845_at

_

No hit

**(-)

   

*(-)

  

Contig7285_at

2.00E-96

Hypothetical protein P0576F08.31(Oryza sativa)

  

*(+)

   

6H (43.15)

Contig7399_s_at

1.00E-115

Ubiquitin carboxyl-terminal hydrolase isozyme L3

 

**(+)

  

**(+)

*(-)

6H (60.23)

HB27E22r_at

_

No hit

    

*(+)

  

HS04A09u_s_at

_

No hit

***(+)

   

*(+)

 

7H (140.21)

HVSMEl0003B06r2_at

_

No hit

**(+)

   

*(+)

  

HVSMEl0007F21r2_s_at

_

No hit

*(+)

      
  1. *, **, ***: Significant correlation at p < .05, p < .01, and p < .001, respectively. +: positive correlation, -: negative correlation. Gene annotations were exported from HarvEST:Barley version 1.68. ME: malt extract, GP: grain protein content, S/T: ratio of wort soluble protein to total malt protein, DP: diastatic power, AA: alpha-amylase activity, BG: malt beta-glucan content. Bold type indicates common genes to both time points. The genetic map locations (map loc.) were based on the barley SNP map [24].