Figure 2

False positive (F+) pool hit rate and false negative (F-) pool hit rate with clone deconvolution. (A) Clone row pool (RP) and column pool (CP) F+ rate; (B) RP and CP F- rate; (C) Row super pool (RSP) and column super pool (CSP) F+ rate; and (D) RSP and CSP F- rate. The F+ rate of a pool for a marker is calculated as F+ hits divided by observed pool hits, and the F- rate of a pool for a marker as F- pool hits divided by true pool hits. Based on a given list of true positive BAC clones associated with a marker, true pool hits of CP, RP, CSP and RSP can be reversely obtained from information extracted from clone names. If a true pool hit is not found in the observed pool hits, the hit is F-. However, if an observed pool hit does not exist in the true pool hits, the hit is F+.