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Table 1 Clone deconvolution of 55 SNP markers mapped on the genetic map of Ae. tauschii chromosome 2D with the 5-D clone pooling strategy.

From: A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening

FPC assembly*

No. of contigs

No. of markers

No. of TP markers

No. of F+ markers

No. of markers without solution

Recall

Precision

1.1

7,447

55

48

4

3

0.87

0.91

1

11,852

55

45

8

2

0.82

0.85

2

17,832

55

8

27

20

0.15

0.23

  1. * (1) Assembly 1: The assembly started at 1×10-15, followed by step-wise DQing down to 1×10-41. The clones resolved by the DQing process were put back into singletons. The singleton-to-contig end merging was performed at 1×10-20. The contig end-to-end merging (requiring a minimum of a 2-clone overlap at each end) at 1×10-25 and at 1×10-15 was performed last. (2) Assembly 1.1: Assembly 1 was subjected to a contig merge. The contig end-to-end merging (requiring only one clone overlap at each end) was performed starting at 1×10-25 and stepping down to the level of 1×10-15. (3) Assembly 2: The clones were assembled into contigs at 1×10-60.
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BMC Genomics

ISSN: 1471-2164

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