A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening

BMC Genomics

Table 2 BAC libraries and clones used in the 5-D clone pooling design

Library code	Cloning site	Vector	No. of clones	No. of plates	Genome coverage	No. of clones in a row sub-pool	No. of clones in a column sub-pool
RI	EcoRI	pECBAC1	54,144	141	1.6×	3,384	2,256
HB	BamHI	pECBAC1	59,904	156	1.6×	3,744	2,496
BB	BamHI	pCLD04541	76,800	200	1.9×	4,800	3,200
HI	HindIII	pECBAC1	59,904	156	1.8×	3,744	2,496
HI	HindIII	pCLD0451	52,224	136	1.6×	3,264	2,176
Total			302,976	789	8.5×	18,936	12,624

Note: only 14 column pools in the HindIII-pECBAC1 library was used in Illumina GoldenGate genotyping.

ISSN: 1471-2164