Library Origin | Selected ASSETs | ASSET validation | Heteroduplex validation | Cross-validation |
|---|
Lib_1
| 6 | 5 | 2 | 4 |
Lib_2
| 7 | 7 | 4 | 7 |
Lib_1 & Lib_2
| 5 | 5 | 0 | - |
- Library Origin: library where the ASSET was captured from; selected ASSETs: number of randomly selected ASSETs; ASSET validation: to confirm the presence of the ASSET in the same RNA used for library construction; heteroduplex validation: amplification of alternatively spliced transcripts that could have participated in the heteroduplex formation; and cross-validation: amplification of ASSETs specifically captured from one library using the cDNA template from the other library.
