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Table 4 Results of the mixed-model analysis of variance (ANOVA) to identify SFPs.

From: A high-density transcript linkage map with 1,845 expressed genes positioned by microarray-based Single Feature Polymorphisms (SFP) in Eucalyptus

Description

SFP-detection by conventional

linkage mapping

SFP-detection by ANOVA at

the probe-set level*

SFP-detection by ANOVA

at the probe level*

# probes on SFP-discovery array

103000

103000

103000

# Genes represented on SFP-discovery array

20726

20726

20726

# Genes selected for SFP-mapping step

15698

4648 (100%)

4251 (100%)

# Probes selected for SFP-mapping step

43777

-

10127 (100%)

# Genes mapped (relaxed marker order)

1845

1603 (87%)

1564 (85%)

# Genes mapped (framework marker order)

884

811 (92%)

797 (90%)

  1. Efficiency in detecting mappable SFP at the gene level (probe-set) and probe level by the ANOVA in comparison to the conventional linkage mapping approach
  2. * In parentheses are indicated the percentage of genes or probes in common with those mapped by conventional linkage mapping considered as the benchmark approach