Figure 3
The non-random chromosomal distribution of several tri- and tetranucleotide SSRs in polytene (panels a, d, e, g, h, i, j, l and o; scale bar 25 μm) and mitotic (b, c, f, k, m, n, p and q; scale bar 5 μm) nuclei of D. melanogaster , as shown by ND-FISH with the indicated biotinylated probes. Each panel shows individual and/or merged images to facilitate the visualization of the red signals with respect to the DAPI (blue) staining and to allow chromosome identification. The view of the localization of the AAT repeats in the base of chromosome X is enlarged in h. A detailed view of the chromocentre (CR) after ND-FISH with (ACT)5, (GACA)4 and (GATA)4 is shown in e and in the inserts in panels l and o respectively. The arrows point to chromosomes X and 4 in b and k respectively.