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Table 3 Agreement between technical replicates for biological replicate 2 D. melanogaster female heads.

From: RNA-seq: technical variability and sampling

 

TR2

TR1

0

1

2

3

4

5

6

7

8

Total

0

9213

2947

3

0

0

0

0

0

0

12163

1

1990

24845

4269

150

0

0

0

0

0

31254

2

0

818

4884

2273

24

0

0

0

0

7999

3

0

16

402

3293

1028

2

0

0

0

4741

4

1

2

2

172

1669

415

2

1

0

2264

5

0

0

0

1

76

749

213

0

0

1039

6

0

0

0

0

0

35

357

108

0

500

7

0

0

0

0

0

0

11

241

9

261

8

0

0

0

0

0

0

0

2

54

56

Total

11204

28628

9560

5889

2797

1201

583

352

63

60277

  1. RPKM was grouped into 7 categories on an approximate log10 scale. The categories were: zero reads, average less than 10, 20, 40, 80, 160, 320, 1000 and greater than 1000. Technical replicates 1 and 2 were compared. Values on the diagonal are where the ordinal categories agree and off diagonal values (bold/italic) are disagreements.