Identification of alternative splice variants in Aspergillus flavus through comparison of multiple tandem MS search algorithms

Table 2 Overlap of identified peptides and proteins between algorithms with a FDR < 2%

Algorithm	Peptides		RefSeq Proteins		Putative Isoforms
	Count	%	Count	%	Count	%
Mascot only	275	7.1	58	5.2	6	8.3
OMSSA only	152	3.9	72	6.5	8	11.1
X! Tandem only	471	12.1	156	14.0	23	31.9
InsPecT only	334	8.6	98	8.8	20	27.8
Mascot, OMSSA	115	3.0	24	2.2	0	0.0
Mascot, X! Tandem	100	2.6	13	1.2	2	2.8
Mascot, InsPecT	80	2.1	10	0.9	1	1.4
OMSSA, X! Tandem	30	0.8	17	1.5	0	0.0
OMSSA, InsPecT	44	1.1	5	0.4	0	0.0
X! Tandem, InsPecT	108	2.8	12	1.1	0	0.0
Mascot, OMSSA, X! Tandem	185	4.8	36	3.2	0	0.0
Mascot, OMSSA, InsPecT	209	5.4	16	1.4	1	1.4
Mascot, X! Tandem, InsPecT	116	3.0	22	2.0	0	0.0
OMSSA, X! Tandem, InsPecT	21	0.5	7	0.6	0	0.0
Mascot, OMSSA, X! Tandem, InsPecT	1651	42.4	568	51.0	11	15.3
Grand Total	3891	100.0	1114	100.0	72	100.0

ISSN: 1471-2164