Figure 4

Dendrograms and histograms for MMP1 in untreated and IM treated HUVECS. Dendrograms of MMP1 RNA expressions are shown for the un-treated (A) and IM-treated (C) HUVECs. Histograms showing the frequency (y-axis) of maximum branch height (x-axis) across 10,000 simulated MMP1 transcript datasets, each with parameters similar to the estimated parameters of the population from which the actual MMP1 data set was drawn, are shown (B and D). Green arrows indicate the maximum branch height from cluster analysis of the actual data sets. In both un-treated and IM-treated HUVECs, the maximum clustering branch height for MMP1 exceeded the maximum clustering branch height identified in 90% of the simulated data sets.