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Table 5 Summary of BLASTX searches to annotated protein databases

From: Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array

Assembly

Contigs hit Uniprot

% contigs with hits

Unique protein hits

Contigs hit zebrafish Refseq

% contigs with hits

Unique zebrafish Refseq hits

Reference

32,350

30.9%

17,766

36,597

34.9%

14,874

De novo

24,168

5.7%

12,331

29,688

7.1%

10,781

Total

56,518

10.7%

24,440

66,285

12.6%

19,899

  1. Contigs of two assemblies, the reference assembly with 104,870 contigs and the de novo assembly with 421,229 contigs, were used to search the Uniprot database and the zebrafish Refseq protein database to assess the number of related genes represented by catfish expressed sequences.