Figure 2

Wrong prediction of snoRNA targets exemplified by rhesus monkey SNORD87 RNA. C, D', C', and D sequences are boxed; the antisense element is marked yellow, and the complementary region in 28S rRNA is shown. The target nucleotide for 2'-O-methylation guided by SNORD87 is indicated by the solid arrowhead. The regions erroneously identified as antisense elements by Zhang et al. [18] are underlined in red. The putative SNORD87 targets identified by Zhang et al. are given below. The only possible SNORD87-guided modification among these targets is indicated by the empty arrowhead. This nucleotide is not methylated in human U6 snRNA.