Skip to main content
Figure 2 | BMC Genomics

Figure 2

From: Functional microRNA screening using a comprehensive lentiviral human microRNA expression library

Figure 2

Transduction efficiencies in different cell systems. (A) A375 cells were infected with different amounts of GFP-encoding virus and different concentrations of polybrene. GFP intensity of each cell was measured after 48 hours and 72 hours. The graphs on the right summarize the GFP intensities of each population in the corresponding row. (B) Transduction efficiency of GFP-encoding virus for several cell lines. For all cell lines tested, transduction efficiencies above 80% were achieved. (C) Successful transduction of human intestinal stem cells with GFP-encoding virus. Infected stem cells were able to grow out into three-dimensional intestinal organoids in Matrigel. Below: magnification of inlay.

Back to article page