Global analysis of gene expression in NGF-deprived sympathetic neurons identifies molecular pathways associated with cell death

Table 1 Genes known to be regulated by NGF withdrawal

Gene symbols	Gene name	-NGF v +NGF	CEP v +NGF	Pathway	References
Bid3/Dp5/Hrk	BH3-interacting domain-containing protein 3; Dp5; harakiri	9.78	5.28	cell death	32, 34
Trib3	tribbles homologue 3 (Drosophila)	5.21	1.81	ER stress	35
Dusp1/Mkp1	dual specificity phosphatase 1;MAP kinase phosphatase 1	4.69	1.60	MAPK pathways	13, 24
Bcl2l11/Bim/Bod	BCL2-like 11 (apoptosis facilitator)	4.35	1.95	cell death	16, 17
Egln3/SM-20	EGL nine homologue 3 (C. elegans)	3.41	3.53	cell death	15
Gadd45γ	growth arrest and DNA-damage- inducible protein 45 gamma	3.16	1.31	DNA damage inducible	33
Jun	Jun oncogene; c-Jun	2.62	1.55	AP1 family	13, 14
Ccnd1	cyclin D1	1.46	1.04	cell cycle	31
Myc	myelocytomatosis viral oncogene homologue (avian); c-Myc	-1.76	-1.32	Myc network	31
Ngfr/p75 ^NTR	nerve growth factor receptor (TNFR superfamily, member 16); p75^NTR	-1.73	-1.44	neurotrophin signalling	31

The fold increase in RNA level after NGF withdrawal or after NGF withdrawal in the presence of CEP-11004 (average of 3 independent experiments) from the microarray is shown for genes that have been previously reported to be transcriptionally regulated in this system (see references above).

ISSN: 1471-2164