Table 1 Functions of the genes involved in methionine utilization
From: The MetJ regulon in gammaproteobacteria determined by comparative genomics methods
gene | function1 |
|---|---|
Regulators | |
metJ | AdoMet-dependent transcriptional repressor |
metR | homocysteine-dependent transcriptional activator |
Biosynthetic enzymes | |
metL | aspartokinase II/homoserine dehydrogenase II |
metA | homoserine O-succinyltransferase |
metX | homoserine O-acetyltransferase |
metY | ~O-acetylhomoserine sulfhydrylase |
metB | cystathionine gamma-synthase |
metC | cystathionine beta-lyase |
metF | 5,10-methylenetetrahydrofolate reductase |
metH | methionine synthase (B12-dependent) |
metE | methionine synthase (B12-independent) |
metK | S-adenosylmethionine (AdoMet) synthetase |
Methionine salvage pathway genes | |
ybdH | ~alcohol dehydrogenase |
mtrA | ~MTR transporter (ATPase subunit) |
mtrC | ~MTR transporter (permease subunit) |
mtrB | ~MTR transporter (substrate-binding subunit) |
mtrY | ~function unknown |
mtnK | 5-methylthioribose (MTR) kinase |
mtnA | S-methyl-5-thioribose-1-phosphate isomerase |
mtnD | 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase |
mtnC | 2,3-diketo-5-methylthio-1-phosphopentane phosphatase |
mtnB | methylthioribulose-1-phosphate dehydratase |
mtnE | methionine aminotransferase |
mtnU | ~nitrilase |
Transporters | |
metN | methionine transporter (ATPase subunit) |
metI | methionine transporter (permease subunit) |
metQ | methionine transporter (substrate-binding subunit) |
metT | ~methionine transporter (Na+/H+ antiporter family) |
btuB | vitamin B12 (cobalamin) outer membrane transporter |
mtsA | ~cobalt transporter (substrate-binding subunit) |
mtsB | ~cobalt transporter (ATPase subunit) |
mtsC | ~cobalt transporter (permease subunit) |
nhaP | ~Na+/H+ antiporter |
Other regulated genes | |
mmuP | S-methylmethionine permease |
mmuM | S-methylmethionine : homocysteine methyltransferase |
MHT1 | ~homocysteine S-methyltransferase |
dppA | ~transporter (substrate-binding subunit) |
dppB | ~transporter (permease subunit) |
dppC | ~transporter (permease subunit) |
dppD | ~transporter (ATPase subunit) |
MHT2 | ~homocysteine S-methyltransferase |
arcD | ~amino acid permease |
metE2a | ~methionine synthetase (B12-independent) |
mtcB | cystathionine beta-synthase |
mtcC | cystathionine gamma-lyase |
megL | ~methionine gamma-lyase |
pcbC | ~oxygenase |
Various unregulated genes | |
ahcY | adenosylhomocysteinease |
asd | aspartate semialdehyde dehydrogenase |
def | peptide deformylase |
fmt | 10-formyltetrahydrofolate : L-methionyl-tRNAfMet N-formyltransferase |
folE | GTP cyclohydrolase I |
glyA | serine hydroxymethyltransferase |
luxS | S-ribosylhomocysteine lyase |
map | methionine aminopeptidase |
metG | methionyl-tRNA synthetase |
mtnN | methylthioadenosine (MTA) nucleosidase/S-adenosylhomocysteine (AdoHcy) nucleosidase |