Figure 4From: Model SNP development for complex genomes based on hexaploid oat using high-throughput 454 sequencing technologySequence validation. Sequencing of real-time PCR amplicons using oat EST-SNP primers revealed both simple (A) and complex (B) polymorphism between Ogle1040 (O) and TAM O-301(T). Individual reads are identified by primer name (c2760_1 and c3768_1) and genotype (O and T). Sequences were aligned using Geneious v4.8.3.Back to article page