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Table 1 Summary of genome features in safe strains.

From: The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli

 

pRK1

pRK2

W

K-12

B

Crooks

Accession & Version

CP002186

CP0021857

CP002185

U00096.2

CP000819.1

CP000946.1

Chromosome size (Kbp)

102.5

5.36

4901

4640

4630

4746

G+C content

49.95

46.03

50.84

50.78

50.77

50.87

genes (pseudogenes)

117 (1)

16 (0)

4764 (91)

4493 (177)

4383 (67)

4409 (82)

CDSs

114

15

4482

4149

4209

4200

structural RNAs

3

1

191

172

107

128

   rRNAs

0

0

22

22

22

22a

   tRNAs (pseudo)

0 (0)

0 (0)

87

89 (3)

85 (0)

87 (1)

   other ncRNAs (pseudo)

2 (0)

1 (0)

82

61 (2)

ND

19

Large Mobile Elements

0

0

10

10

11

9

   Prophage regions

0

0

7

8

10

8

   Integrative Elements

0

0

3

2

1

1

IS elements (pseudo)

2 (0)

0 (0)

18 (6)

41 (13)

50 (12)

39 (15)

LPS core type

-

-

R1

K-12

R1 (IS1::waaT)

R1

O antigen

-

-

O6

O16 (IS5::wbbL)

O7 (IS1::wbbD)

O146 (IS1::wbwW)

H antigen

  

H49

H48

-

ND

K antigen

-

-

-

-

K5 (IS1::kfiB)

-

Colanic acid (M-antigen)

-

-

+

+

+

+

  1. a ssrS is annotated as an rRNA in Crooks but in K-12 and W it is annotated as an ncRNA. It is included in this table as an ncRNA
  2. The total number of genes, tRNA, other ncRNAs and IS elements in each strain includes pseudogenes/pseudo-tRNAs etc.; the number of pseudo-elements in each case is in noted in brackets. A '+' means the element is present; a '-' means the element is absent. ND = not determined in annotation. Safe laboratory strains: W (ATCC 9637) and its plasmids pRK1 and pRK2; K-12 (MG1655); B (REL606); and Crooks (ATCC 8739). W is in phylogroup B1; K-12, B and Crooks are in phylogroup A.