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Table 1 Comparison of results from different NGS assemblers

From: In silico secretome analysis approach for next generation sequencing transcriptomic data

Assembler

No. of second order contigs

No. of contigs

Largest contig

Average length

N50*

N90*

Number of bases

MIRA [8] + CAP3 [29]

3056

25845

3620

402.36

406

253

11628536

Newbler [26]

 

25127

2607

407.11

409

252

10229510

  1. *N50 refers to the length of the shortest contig such that the sum of contigs of equal length or longer is at least 50% of the total assembly size. While N90 refers to the length of the shortest contig such that the sum of contigs of equal length or longer is at least 90% of the total assembly size.
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BMC Genomics

ISSN: 1471-2164

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