Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomes

BMC Genomics

Table 1 Average GC content

Sample	Library	PCR-free	Kapa HiFi	Kapa2G	AccuPrime	Platinum	RPA	T7	Phusion
3D7	Av. %GC content	19.50	20.35	21.44	21.93	21.47	20.75	22.63	23.80
PK0076	Av. %GC content	21.92	19.79	21.07	21.37	21.91	19.66	20.95	22.87

Calculated values of average GC content corresponding to each library preparation. Deviation from the 19.4% (value of the unamplified genome) indicates the effect of amplification bias. See Figure 3 A & B for a global graphical presentation of the GC content distribution. 3D7, P. falciparum strain 3D7; PK0076, P. falciparum clinical isolate.

ISSN: 1471-2164