Table 5 PCR primers used to amplify the mitochondrial genomes of Nectonemertes cf. mirabilis
Primer name | Sequence (5' → 3') | References |
|---|---|---|
Universal | Â | Â |
rrnS-rrnL | Â | Â |
N12SF | TGTGCCAGCTTCCGCGGTTATAC | Present study |
N16SR | ACGCTGTTATCCCTATGGTA | Present study |
rrnL-cob | Â | Â |
16SarL | CGCCTGTTTATCAAAAACAT | [57] |
CytbR | GCRTAWGCRAAWARRARTAYCAYTCWGG | [58] |
Nectonemertes cf. mirabilis | Â | Â |
cox1 | Â | Â |
LCO-1490 | GGTCAACAAATCATAAAGATATTGG | [59] |
HCO-2198 | TAAACTTCAGGGTGACCAAAAAATCA | [59] |
cox3 | Â | Â |
cox3F | TGCGWTGAGGWATAATTTTATTTATT | [8] |
cox3R | ACCAAGCAGCTGCTTCAAAACCAAA | [8] |
cob-cox3 | Â | Â |
Nm cobF | TCGGTGGATAATGCTACTTTG | Present study |
Nm COX3R | ACCAGAAGCCAACAATACAGC | Present study |
cox3-cox1 | Â | Â |
Nm COX3F | TGTTGGCTTCTGGTGTTAGTG | Present study |
Nm COX1R | GAGCCTCTTTCAACAACAGCA | Present study |
cox1-rrnS | Â | Â |
NmCOX1F | AATCTGGTCTGGGTTGGTTGGCACTGCGTTA | Present study |
Nm12SR | GACTCCCCTGAAAGGACATAAAACACCG | Present study |
Zygeupolia rubens | Â | Â |
cob-cox3 | Â | Â |
Zrcob F | CTTTGGGTTTGTTGCTGTTG | Present study |
ZrCOX3R | GTTGAACCATAAATCCCATC | Present study |
cox3-cox1 | Â | Â |
cox3F | TGCGWTGAGGWATAATTTTATTTATT | [8] |
ZrCOX1R | GAGCCTCTTTCAACAACAGCA | Present study |
cox1-cox2 | Â | Â |
LCO-1490 | GGTCAACAAATCATAAAGATATTGG | [59] |
NCOX2R | AAAGAATGATTWGCWCCAC | Present study |
cox2-rrnS | Â | Â |
ZrCOX2F | TTTGGCTTTACCTTCTTTGC | Present study |
Zr12SR | AAATAAGACACCGCCAAGT | Present study |