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Figure 1 | BMC Genomics

Figure 1

From: Generation and analysis of a barcode-tagged insertion mutant library in the fission yeast Schizosaccharomyces pombe

Figure 1

A non-homologous recombination-mediated insertion mutagenesis for generating an S. pombe mutant library. (A) The first insertion vector tested had the selectable marker ura4+ and a 15-bp random barcode directly following the 3’ UTR of ura4+. (B) The insertion vector used to construct the S. pombe insertion mutant library is composed of a selectable marker ura4+ gene, a barcode (27 random nucleotides with 14 interspersed A’s), a lox71 site for one-way integration of lox66-bearing DNA, a mutated human HSP70 promoter with a lexA binding site and a modified λ phage sequence, ATG-less λ, to protect the sequences 3’ to the λ phage fragment from degradation.

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