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Figure 2 | BMC Genomics

Figure 2

From: Generation and analysis of a barcode-tagged insertion mutant library in the fission yeast Schizosaccharomyces pombe

Figure 2

Ligation-mediated barcode oligomerization. (A) Barcodes in this insertion mutant library can be amplified from a population of mutants by PCR using vector-specific primers that flank barcodes. Overhangs generated by Sfi I digestion allow barcode monomers to be oligomerized in a head-to-tail manner. (B) A representative barcode oligomerization. The lanes labeled “monomer” and “oligomers” show the Sfi I-digested barcode DNA before and after ligation, respectively.

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BMC Genomics

ISSN: 1471-2164

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