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Figure 4 | BMC Genomics

Figure 4

From: Distribution of nitrogen fixation and nitrogenase-like sequences amongst microbial genomes

Figure 4

Maximum-likelihood phylogenetic tree of conventional nitrogenases and nitrogenase-like sequences. The tree is represented by a core set of 73 sequences, selected from a larger tree of 472 sequences. Shimodaira-Hasegawa local support values were >0.6 except for those nodes marked with a red star. The clade coloring reflects sequences that are co-located in genomes and likely to correspond to the alpha and beta subunits of nitrogenase, with the exception of those shown in light gray, which are single subunit enzymes (NflD). Dark blue clades are conventional nitrogenases, labeled as NifD/E and NifK/N respectively. Clades colored in light-green are NifD/E and NifK/N-like sequences in which the FeMoco ligand Cys 275 in the alpha component, is either present (dark green nodes) or absent (yellow nodes). In all other cases known FeMoco ligands are absent. The number of conserved Cys residues in each subunit that correspond to P cluster ligands in conventional nitrogenases are indicated for each clade.

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