Pharmacological gain of function of the Shh/Gli pathway activates transcription of novel target genes. A) C3H10T1/2 cells were cultured for 48 h in 10 μM pur or its control (vehicle DMSO) and processed for qPCR. c-myc shows significant differences in mRNA levels, whereas sfrp2 does not. ptc 1 is shown as a positive control. B) neo1 mRNA levels are increased after the same 48 h treatment of C3H10T1/2 cells, graph showed separately due to use of TaqMan probes.