Table 2 Primers and restriction enzymes used in generating 21 SBP markers
Ch. No. | Name | Primer sequence | Restriction enzyme | Amplicon size (bp) |
|---|---|---|---|---|
1 | SBP1_0.95 | F:GTCAGGCTAGCTCATCAAGTCCTAC R:CCGGATTTCGCCAGCTCCGTC | Tsp 509I | 100 |
1 | SBP1_5.25 | F:CACAAACCCTTCACCTCCAT R:GCAGTTGCCTAAAGGCTGAG | Msp I | 233 |
1 | SBP1_8.08 | F:AACGCAATTCTCAAGCAGGT R:CATTCAATTGTTGGGCAGTG | Rsa I | 188 |
1 | SBP1_11.09 | F:AAAGTCAACCGGGAGGTTTC R:AGGCTGAGGACACGAGAAGA | Bam HI | 163 |
1 | SBP1_18.63 | F:GCACTTGCAAAAGGAAGCTC R:TTCTTGCTGGAGAATCGTGA | Rsa I | 197 |
1 | SBP1_22.30 | F:TACCGGTTCCGGTCACTATC R:AATGGGAAATTGGGATTGGT | Dde I | 151 |
1 | SBP1_25.92 | F:TTGTTGAGAGAGCGAGATCAAA R:AAAAGCATCACATCATCTTTGG | Nhe I | 102 |
2 | SBP2_14.07 | F:GAAGGAATTGGACCAAACGA R:ATCTAGCTGCCCTCACTGGA | Bts CI | 213 |
2 | SBP2_16.23 | F:CACCATTTGTTCCCGTAAGC R:TGGTCAATCCATGGTGATGT | Hpy 166II | 157 |
3 | SBP3_6.60 | F: CCATCGTCCTATTCTAATCCATGTTG R: GATGCAAAATCTCCATCCTCTTC | Tsp 509I | 379 |
3 | SBP3_8.11 | F:CACGTATCGGCGAGTCTACA R:CAAATTCAAATCTCAGTTTTCGTC | Taq I | 150 |
3 | SBP3_10.28 | F:TCTAAAACGAACCGGGAAAA R:CGACAAGTAAATTAAAACCAACCTG | Mbo II | 151 |
3 | SBP3_11.12 | F:AAGACTTTGGTTCAACTCCTGAA R:GGCTTTGGATTCAGGAAAAA | Taq I | 184 |
3 | SBP3_23.46 | F: CGACCAAATGTCTCTGAGATGTTC R: CACCCAAGGCGGTGTTGGCGAAAG | Taq I | 520 |
4 | SBP4_0.67 | F:CGGTTAACATGCCTCAATCC R:TGTGGATGATTTGGGGACTC | Dde I | 171 |
4 | SBP4_2.91 | F:CGAGTGACTTCTTGAGGTTTATTATG R:CGAGATTGCTTTGGTATGGA | Hpy 166II | 249 |
4 | SBP4_5.60 | F:AGGGAAGAATATGCGGAAGG R:TGTTTCTGTCTTGGCCCATT | Taq I | 159 |
4 | SBP4_6.51 | F:GGACAAGACCTTGATTTGAAGTTTG R:GAGGGCTCACATTGGGTTTAATG | Tsp 509I | 395(C), 490(N) |
5 | SBP5_8.40 | F:TCGACGGTGACTTGTAGGTG R:CGATGCCGTCTCATAAAAGG | Dpn I | 232 |
5 | SBP5_14.60 | F: CGCGGTTATGGTAACGTTAAATG R: CCGAGGGAGAAGAAAGGATCAAGAAG | Hph I | 225 |
5 | SBP5_25.00 | F:AAATCACCAATGGCAAAACA R:TTTGCGTAGACGGAGAGTGA | Dde I | 191 |