- Research article
- Open Access
Genomic determinants of organohalide-respiration in Geobacter lovleyi, an unusual member of the Geobacteraceae
© Wagner et al.; licensee BioMed Central Ltd. 2012
- Received: 14 November 2011
- Accepted: 22 May 2012
- Published: 22 May 2012
Geobacter lovleyi is a unique member of the Geobacteraceae because strains of this species share the ability to couple tetrachloroethene (PCE) reductive dechlorination to cis-1,2-dichloroethene (cis-DCE) with energy conservation and growth (i.e., organohalide respiration). Strain SZ also reduces U(VI) to U(IV) and contributes to uranium immobilization, making G. lovleyi relevant for bioremediation at sites impacted with chlorinated ethenes and radionuclides. G. lovleyi is the only fully sequenced representative of this distinct Geobacter clade, and comparative genome analyses identified genetic elements associated with organohalide respiration and elucidated genome features that distinguish strain SZ from other members of the Geobacteraceae.
Sequencing the G. lovleyi strain SZ genome revealed a 3.9 Mbp chromosome with 54.7% GC content (i.e., the percent of the total guanines (Gs) and cytosines (Cs) among the four bases within the genome), and average amino acid identities of 53–56% compared to other sequenced Geobacter spp. Sequencing also revealed the presence of a 77 kbp plasmid, pSZ77 (53.0% GC), with nearly half of its encoded genes corresponding to chromosomal homologs in other Geobacteraceae genomes. Among these chromosome-derived features, pSZ77 encodes 15 out of the 24 genes required for de novo cobalamin biosynthesis, a required cofactor for organohalide respiration. A plasmid with 99% sequence identity to pSZ77 was subsequently detected in the PCE-dechlorinating G. lovleyi strain KB-1 present in the PCE-to-ethene-dechlorinating consortium KB-1. Additional PCE-to-cis-DCE-dechlorinating G. lovleyi strains obtained from the PCE-contaminated Fort Lewis, WA, site did not carry a plasmid indicating that pSZ77 is not a requirement (marker) for PCE respiration within this species. Chromosomal genomic islands found within the G. lovleyi strain SZ genome encode two reductive dehalogenase (RDase) homologs and a putative conjugative pilus system. Despite the loss of many c-type cytochrome and oxidative-stress-responsive genes, strain SZ retained the majority of Geobacter core metabolic capabilities, including U(VI) respiration.
Gene acquisitions have expanded strain SZ’s respiratory capabilities to include PCE and TCE as electron acceptors. Respiratory processes core to the Geobacter genus, such as metal reduction, were retained despite a substantially reduced number of c-type cytochrome genes. pSZ77 is stably maintained within its host strains SZ and KB-1, likely because the replicon carries essential genes including genes involved in cobalamin biosynthesis and possibly corrinoid transport. Lateral acquisition of the plasmid replicon and the RDase genomic island represent unique genome features of the PCE-respiring G. lovleyi strains SZ and KB-1, and at least the latter signifies adaptation to PCE contamination.
- Genomic Island
- Cobalamin Biosynthesis
- Organohalide Respiration
- Customize Perl Script
- CxxCH Motif
Geobacter spp. are common members of anoxic freshwater sediment and subsurface microbial communities, where they are involved in the reduction of oxidized metal species and the turnover of organic matter . Members of this genus show promise for bioremediation of anoxic subsurface environments contaminated with toxic radionuclides . While dissimilatory metal reduction is a hallmark feature of Geobacter, the ability to use chlorinated organic compounds as electron acceptors has only recently been discovered in this genus, and appears to be restricted to a single Geobacter clade (Additional file 1) with only a few cultured representatives . Organohalide respiration has been described for Geobacter thiogenes strain K and Geobacter lovleyi strain SZ, which dechlorinate trichloroacetate to dichloroacetate and tetrachloroethene (PCE) to cis-1,2-dichloroethene (cis- DCE), respectively [3, 4]. In addition, another PCE-to-cis- DCE-dechlorinating G. lovleyi strain, designated strain KB-1, was identified in the PCE-to-ethene-dechlorinating consortium KB-1 . G. lovleyi 16S rRNA gene sequences have been detected at the contaminated Oak Ridge IFRC site  and in trichloroethene (TCE)-contaminated sediments from Ft. Lewis, WA . A recent continuous flow column study using the PCE-to-ethene-dechlorinating bioaugmentation consortium Bio-Dechlor INOCULUM (BDI) containing G. lovleyi strain SZ indicated that PCE-dechlorinating Geobacter strains enhance dissolution of free phase PCE . Further, G. lovleyi strain SZ uses graphite electrodes as a direct electron donor for reductive dechlorination (organohalide respiration), possibly enabling innovative bioremediation approaches . The organohalide-respiring Geobacter strains share 16S rRNA gene sequences with 98–100% identity to each other but only 93% identity with G. sulfurreducens strain PCA, the type strain for the Geobacter genus. Among the δ-Proteobacteria Desulfuromonas michiganensis is the only other PCE-to-cis-DCE-respiring species but genome information is not available . The genus Anaeromyxobacter (δ-Proteobacteria) comprises isolates with sequenced genomes, which are capable of using chlorinated phenols as respiratory electron acceptors, but PCE dechlorination has not been reported [11–13].
Due to its implications for bioremediation of sites contaminated with both chlorinated ethenes and radionuclides , and the paucity of genome information of PCE-respiring δ-Proteobacteria G. lovleyi strain SZ is a promising reference strain for understanding the physiological and evolutionary responses of microbes to anthropogenic changes in the environment. Here we present the G. lovleyi strain SZ genome sequence, including the discovery of a unique 77 kbp plasmid (pSZ77). The plasmid is notable, as it contains laterally acquired genes and some that encode functions important to Geobacter metabolism such as cobalamin biosynthesis. Comparative analyses of the strain SZ genome revealed significant functional divergence from previously sequenced Geobacter spp. and mobile elements sharing genomic features of Pelobacter, a distinct genus of the δ-Proteobacteria.
Cultures and growth conditions
G. lovleyi strain SZ , G. lovleyi strain KB-1 (AY780563) , G. thiogenes, and four G. lovleyi isolates Geo7.1, Geo7.2, Geo7.3, and Geo7.4 obtained from PCE-to-cis-DCE-dechlorinating microcosms established with Fort Lewis, WA, soil  were utilized in this study. G. lovleyi strain KB-1 was isolated from consortium KB-1. Following a series of eight 1:20 vol/vol dilution transfers to defined mineral medium supplemented with 90 mg L−1 PCE and 10 mM acetate, the final dilution culture was used to inoculate a set of serial dilution agarose shake tubes amended with 10 mM fumarate and 10 mM acetate . Transfer of a colony from a 10−2 dilution tube to liquid culture yielded a pure culture of G. lovleyi strain KB-1. In addition, the PCE-to-ethene-dechlorinating consortia BDI and KB-1  were grown and maintained with 0.05 mg mL−1 PCE as electron acceptor. Pure and mixed cultures were grown in 60 mL serum bottles containing 40 mL reduced, defined mineral salts medium with 5 mM acetate serving as both carbon source and electron donor with an N2-CO2 (80:20, vol/vol) headspace . Lactate (5 mM) substituted for acetate to grow G. thiogenes and consortium BDI. The KB-1 consortium was maintained with methanol as an electron donor, amended to 5 times the electron equivalents required for complete dechlorination. To test the effects of cyanocobalamin (CN-Cbl) on PCE dechlorination, cultures of strain SZ were amended with 0.05 mg mL−1 PCE as the sole electron acceptor and 0, 15, and 750 μg CN-Cbl L−1. Chlorinated ethenes were quantified by gas chromatography as described [17, 18].
To explore if plasmid pSZ77 was stably maintained (i.e., segregational stability), strain SZ cultures were grown at 35°C with 20 mM sodium fumarate as the electron acceptor in medium with 1,500 μg L−1 and without CN-Cbl. When visible turbidity was apparent after 24 to 72 hours, the cultures were consecutively transferred (1% inoculum size, vol/vol) for at least 20 times to fresh medium. Plasmid curing was also attempted by amending liquid cultures with 0.001–0.1% SDS (w/vol)  or 0.1 M L-ascorbic acid .
Genomic DNA extraction and PCR
PCR primers were designed using Primer3 software (http://frodo.wi.mit.edu) based on the predicted open reading frame (ORF) encoding the homolog to the replication initiation protein RepA identified on pSZ77. The primers repA_136F (5'-AGCATCGGTCAGCTGAATCT-3'), and repA_700R (5'-GGTTAGAGCGTGGTGCATTT-3') were used to amplify a 565 bp fragment of the pSZ77 repA gene. To test for the presence of repA in other cultures, biomass was collected from 2 mL aliquots of pure and mixed cultures by centrifugation at 13,200 rpm for 20 min at room temperature (RT). The pellet was added to a bead tube and the DNA extracted according to the protocol for the PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc.) provided by the manufacturer. PCR reactions were prepared in a volume of 15 μL containing 1x PCR buffer, 1.5 mM MgCl2, 200 μM dNTPs, 200 nM of each forward and reverse primers, 0.02 U of GoTaq DNA polymerase and 1 μL of DNA template. The PCR thermocycler program for the repA-targeted primers was 94°C for 2 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 56°C for 30s, extension at 72°C for 30s, and a final extension at 72°C for 6 min. PCR was also carried out using the primer pair Geo564F/840R  that targets the 16S rRNA gene of all Geobacteraceae. PCR conditions and the amplification profile used for the Geo564F/840R set were as described for the repA primer set.
For large plasmid isolation using a modified protocol based on the Kieser method [22, 23], 50 to 200 mL of fumarate-grown SZ culture and 100 mL each of fumarate-grown G. thiogenes and the four Fort Lewis Geobacter isolates were collected by centrifugation (3,220 x g, 30 min). The DNA was isolated as previously described except that the plasmid DNA was precipitated by standard methods  and suspended in a final volume of 50–100 μL TE buffer (10 mM Tris, 1 mM EDTA [pH 8.0]). Plasmid DNA extracts were separated by electrophoresis through a 0.7% (w/vol) agarose Tris-acetate gel run in 1x Tris-acetate EDTA (TAE) buffer . DNA was visualized by staining in 0.5 μg ethidium bromide per mL TAE buffer solution. Isolate G. lovleyi strain KB-1 was grown with 10 mM acetate and 90 mg L−1 PCE, and plasmid DNA was extracted from biomass obtained from 1 L of culture suspension using the Qiagen Plasmid Midi Kit and the modified protocol for large inserts (http://www.qiagen.com/literature/handbooks/default.asp).
Genomic DNA of G. lovleyi strain SZ was extracted from cells grown with acetate and fumarate following established protocols (Bacterial genomic DNA isolation using CTAB. http://my.jgi.doe.gov/general/). Sequencing was performed by the Department of Energy’s Joint Genome Institute (JGI) using a combination of 454 and Sanger reads with average lengths of 107 and 949 bp, respectively. The total 662,511 reads provided an average 25-fold coverage for the chromosome and 40-fold coverage for the plasmid. The G. lovleyi strain SZ genome sequences have been assigned GenBank accession numbers CP001089 and CP001090 for the chromosome and pSZ77, respectively. DNA was extracted from consortium KB-1 following an established protocol , and clone libraries were generated by the JGI using in-house protocols (http://www.jgi.doe.gov/sequencing/protocols) and sequenced by the Sanger approach. The strain KB-1 plasmid DNA was incorporated into a bar-coded 454 GS FLX Titanium sequencing run at the Center for Applied Genomics at the University of Toronto. The 454 reads, along with the consortium KB-1 metagenome contigs identified as Geobacter plasmid sequences, were aligned against pSZ77 using the Geneious assembly tool (http://www.geneious.com). Metagenome reads with disagreements with the pSZ77 sequence were verified and removed if necessary. Read depth was at least 3-fold for most of the assembly with a maximum of 12-fold coverage, but as low as single coverage in two regions of less than 136 bp. Amplicons of the 16S rRNA genes of the Ft. Lewis G. lovleyi strains Geo7.1, Geo7.2, Geo7.3, and Geo7.4 were sequenced twice each by the Sanger approach and assigned the GenBank accession numbers JN982204 through JN982211.
Customized Perl scripts were used to determine GC percentage of all predicted protein-coding genes on the strain SZ chromosome and pSZ77 with standard deviations from genomic averages computed in R (http://www.r-project.org/). COGs assignments were determined from the NCBI .ptt files for the Geobacteraceae and related non-Geobacteraceae chromosomes (AE017180, CP000148, CP000698, CP001390, CP001124, CP001661, CP002479, CP000482, and CP000142) and plasmids (CP000149 CP000483, and CP000484) using customized Perl scripts. Candidate c-type cytochromes were determined by searching all strain SZ amino acid sequences for the CxxCH motif using a customized Perl script, and then further screened for homology to c-type cytochromes in the RefSeq database using PSI-Blast (e-value = 1e-11, h = 1e-5)  and the PROSITE profile for c-type cytochromes (ca.expasy.org/tools/scanprosite/). CxxCH-containing sequences lacking over 50% of PSI-Blast matches annotated as c-type cytochromes or lacking recognizable c-type cytochrome PROSITE profiles (i.e., PS51007, PS51008, PS51009, and PS51010) were eliminated from further analysis. Trans-membrane regions of predicted outer membrane receptor proteins were determined using Pred-TMBB . The nucleotide sequence of pSZ77 and the chromosomal genomic islands on the SZ chromosome were analyzed for repeats using REPuter . Codon adaptation indices (CAI) for strain SZ ORFs encoded on the plasmid and chromosomal genomic islands were computed against a codon usage table based upon all strain SZ chromosomal ORFs using the E-CAI server . Computed CAI were normalized to the expected CAI based upon a 5% level of significance for the bootstrapped set of all ORFs on the SZ chromosome, such that putative foreign genes (i.e. non-Geobacter) would score < 1.00 . The origin of replication of pSZ77 was identified using Ori-Finder , which searches for DnaA-binding sites at regions of GC-skew reversals. The amino acid sequences of replication initiation genes (repA) were used to infer plasmid phylogeny and 16S rRNA genes DNA sequences were used to infer genome phylogeny. All alignments were performed in MUSCLE  and used to build trees in Phylip with topology inferred by bootstrapped neighbor-joining and branch lengths computed by maximum likelihood . 16S rRNA gene sequences were also used to confirm family and genus affiliation using the Ribosomal Database Project . Trees were visualized and formatted using the interactive Tree of Life tool .
Translations of all predicted GenBank ORFs on pSZ77 were run through BlastP  against the nr database with BLOSUM45 and an e-value cutoff of 0.00001. To determine pSZ77 genes and chromosomal genomic islands acquired from bacteria outside of the Geobacteraceae, all translated ORFs on pSZ77 and the SZ chromosome were queried using BlastP against the genomes of Geobacter sulfurreducens strain PCA (AE017180), Geobacter metallireducens strain GS-15 (CP000148), Geobacter uraniireducens strain Rf4 (CP000698), Geobacter sp. strain FRC-32 (CP001390), Geobacter bemidjiensis strain Bem (CP001124), Geobacter sp. strain M21 (CP001661), Geobacter sp. strain M18 (CP002479), Pelobacter propionicus DSM 2379 (CP000482), and P. carbinolicus DSM 2380 (CP000142). Pairwise average amino acid identities between predicted proteomes were mined from BlastP output using custom Perl scripts and genome affiliations of top BlastP matches were checked against IMG statistics for G. lovleyi strain SZ (img.jgi.doe.gov). Sequence similarities to orthologs for pSZ77 cobalamin biosynthesis genes, pSZ77 repA, the chromosomal pce-genes, and chromosomal conjugative pilus tra-genes were determined using PSI-Blast . Further functional inference of amino acid sequences from plasmid-or genomic-island-encoded genes were performed by parsing for conserved domains in PFAM (pfam.janelia.org) and conserved motifs in PROSITE (ca.expasy.org/tools/scanprosite/). A physical map of pSZ77 was plotted using CGview .
The Geobacter lovleyi strain SZ genome
General features of the Geobacter lovleyi strain SZ chromosome
Total number of predicted protein-coding genes
Number of pseudogenes
Total intact protein-coding genes
- matching annotated homologs
- matching hypotheticals only
- with no public database matches
- in one or more COGs
- in energy production COGs
- in coenzyme metabolism COGs
- as predicted c-type cytochromes
- percent with top BlastP match in Geobacter spp.
- percent with top BlastP match in Pelobacter spp.
- percent with top BlastP match in other δ-Proteobacteria
Number of rRNA operons (16S-23S-5S)
Number of transfer RNAs
General features of the G. lovleyi strain SZ plasmid pSZ77
Total number of protein-coding genes
Number of pseudogenes
Number of intact protein-coding genes
- matching annotated homologs
- matching genes with hypothetical function only
- with no public database matches
- in one or more COGs
- in energy production COGs
- in coenzyme metabolism COGs
- predicted as c-type cytochromes
- percent with top BlastP match in Geobacter (7 genomes)
- percent with top BlastP match in Pelobacter (2 genomes)
- percent with top BlastP match in other δ-Proteobacteria
Number of rRNA operons (16S-23S-5S)
Number of transfer RNAs
The six pce-genes of the G. lovleyi genome are homologous to genes in functionally characterized PCE respiration gene clusters. Functions for three of the four components of the pce-gene cluster, pceA-pceB-pceC-pceT, from the Firmicutes (Figure 1B) is supported by experimental evidence in Desulfitobacterium hafniense strains TCE1  and Y51  and Dehalobacter restrictus strain PER-K23 . pceA encodes the catalytic PceA RDase subunit, whose activity towards organohalides is dependent upon a bound cobalamin cofactor . pceB is inferred to encode a membrane-bound subunit to PceA, but direct experimental evidence for this function is lacking. pceC is co-transcribed with pceA-pceB in D. hafniense Y51 and the PceC protein is believed to function in regulating pceA gene expression and electron transfer to the PceA protein . pceT encodes a protein shown to function as a chaperone to the PceA preprotein . The order of the predicted G. lovleyi pce-genes, pceT-pceC-pceA1-pceB1-pceA2-pceB2, differs from that of the functionally-characterized pce-gene clusters (Figure 1B). The G. lovleyi pceA-pceB ORFs, encoding the predicted PCE RDase catalytic subunit and the membrane anchor subunit, respectively, are duplicated on tandem 2,466 bp long blocks sharing 99.8% nucleotide identity (Figure 1A). Despite apparent differences in organization, the amino acid sequences encoded by all six G. lovleyi pce-genes have their most similar homologs among the functionally characterized pce-gene clusters from Desulfitobacterium hafniense strain Y51 (AB070709), D. hafninese strain TCE1 (AJ439608), and D. restrictus (AJ439607). Both sets of strain SZ’s putative PceA and PceB proteins share 33–36% amino acid (aa) identity with PceA (RDase A subunit, BAE84628) and PceB (RDase B subunit, BAE84627) from D. hafniense Y51 . The G. lovleyi PceAs share only distant similarity with the genomic-island-encoded VcrA RDase of Dehalococcoides sp. strain VS (18% identity, 30% similarity) . Glov_2869 (putative PceC) shares a FMN-binding domain (pfam04205), a polyferredoxin (COG0348) domain, and 33% aa identity with the D. hafniense Y51 PceC regulatory protein (BAE84626). Glov_2868 (putative PceT) is annotated as 'peptidylprolyl isomerase' and shares 19% aa identity with PceT from D. hafniense (BAE84625). Despite their apparent shared conserved functions, the low sequence similarities and lack of synteny between strain SZ and the Desulfitobacterium spp. pce-gene clusters suggests the gene clusters have diverged from their shared ancestral gene cluster over time.
An IS21-like integrase gene cluster and a transposase gene flank the strain SZ pce-genes upstream and downstream, respectively, but neither exhibits the compositional features suggestive of recent lateral gene transfer, with GC content, codon biases, and GC-skew consistent with the G. lovleyi genome. Transposase-associated repeats were found to mediate circularization, and presumably excision, of the D. hafniense strain TCE1 PceA-encoding transposon . In contrast, no repeats flanking the SZ PceA genomic island could be detected, and the ISL3-superfamily transposase (pfam01610) downstream of the SZ pce-genes lacks detectable similarity with the mutator family transposases (pfam00872) found on the D. hafniense strain TCE1 transposable element. Furthermore, the rve family integrase (pfam00665) and IS21-type ATPase/integrase cluster upstream of the SZ pce-genes, along with adjacent non-coding DNA (Figure 1A), do not share homology with the dsiB integrase in the vcrA genomic islands of Dehalococcoides nor any other mobilization-related genomic elements (inverted repeats, direct repeats, etc.) associated with RDase genes . The integrase and the ATPase instead appear to originate from within the Geobacter genus, sharing 62% and 80% aa identity, respectively, with their homologs in G. uraniireducens. The SZ Pce genomic island integrase and transposase lack homology with known RDase-associated mobile elements, while the GC content of the pce-genes suggests much of this element was acquired by strain SZ relatively recently. The apparent divergence of the strain SZ pce-genes from their closest known orthologs in Desulfitobacterium spp. suggests the SZ genes are not a recent lateral transfer from the Desulfitobacterium/Dehalobacter group but from another, yet unidentified donor.
The tra-gene cluster, Glov_0304 to Glov_0322, encodes proteins homologous to known plasmid-encoded DNA-transfer F-type conjugative pili . Out of the 19 proteins encoded on the tra-gene cluster, five have PSI-Blast matches to genes unique to F-type conjugative pili (i.e., traN traW, and traU), while seven have PSI-Blast matches to conserved F-type and P-type conjugative pilus “core” genes: traE traL traK traB traV traC, and traG[51, 52] (Figure 2). All but one of the proteins on the strain SZ putative F-type pilus cluster share synteny and high similarity with genes on the chromosome of P. propionicus (sharing 40–91% aa identity) and the 202 kbp plasmid pPRO1 of P. propionicus (37–61% aa identity) (Figure 2). Nearly half of the 19 genes in the strain SZ conjugative pilus cluster have GC contents near the strain SZ genomic average and normalized CAI > 1.00 (Additional file 4), suggesting a sufficient residence time on the SZ genome to ameliorate to SZ chromosomal codon usage . Four additional genomic islands on the SZ chromosome are predicted based on low %GC, disruption of GC-skew, and/or the lack of homologs in a majority of other Geobacter spp. genomes. These additional inferred genomic islands, hyp1, hyp2, and hyp3 encode hypothetical proteins and proteins assigned to COGs class L or class V, while genomic island M contains five genes predicted to function in capsule polysaccharide biosynthesis (Additional file 3).
Electron transfer and oxidoreductases
Predicted energy metabolism genes identified on the genomes of Geobacter spp. and Pelobacter propionicus.
Protein-coding genes in energy production COGs (class C)
Number of predicted c-type cytochromes
c-type cytochromes with >10 heme-binding motifs
The reduced set of multi-heme c-type cytochromes in strain SZ (Table 3, Additional file 5) appears adequate in mediating respiration with U(VI), Mn(IV), and Fe(III) oxides  and electrode surfaces . Specific functions have only been determined for cytochromes with 10 or fewer hemes . Decaheme c-type cytochromes have been confirmed to mediate respiration on electrodes in Shewanella and an 8-heme outer-membrane cytochrome has been shown to be involved in electron transfer to solid state electrodes in Geobacter sulfurreducens. Smaller outer membrane c-type cytochromes with 4–6 hemes are essential for Geobacter respiration with insoluble Fe(III) and Mn(IV) oxides . PilA-type pilins may play an essential role in metal reduction , particularly in radionuclide reduction . Strain SZ encodes a putative PilA (Glov_2096) sharing 81% aa identity with the conductive pilus protein (PilA) of G. sulfurreducens (GSU1496) . P. propionicus also encodes a putative PilA (Ppro_1656), which shares 80% identity with the G. sulfurreducens PilA. Yet, P. propionicus has a reduced repertoire of c-type cytochromes, and is completely lacking decaheme c-type cytochromes (Additional file 6). Accordingly, P. propionicus lacks the ability to respire on electrodes or radionuclides . By contrast, the strain SZ genome carries the predicted minimal set of genes to allow utilization of a comparable range of electron acceptors as other Geobacter spp. .
Additional genes that are key to Geobacter electron transfer are present as multiple paralogs on the strain SZ genome. The strain SZ genome encodes 11 molybdopterin oxidoreductase domain proteins (pfam00384), a larger number compared to other Geobacter genomes. The strain SZ molybdopterin oxidoreductase-type proteins, for which function can be inferred, include three nitrate reductases and two formate dehydrogenases (Additional file 7). One of the nitrate reductase proteins is a periplasmic-type, sharing 43% aa identity (61% similarity) with the respiratory nitrate reductase (NapA) from Desulfovibrio desulfuricans. The role of the two predicted formate dehydrogenases is unclear, as strain SZ does not utilize formate as an electron source or carbon source under PCE- or Fe(III)-reducing conditions . The SZ chromosome encodes seven fumarate reductase/succinate dehydrogenase-type flavoproteins (pfam00890), while no other Geobacter genome encodes more than three. Strain SZ uses fumarate as an electron acceptor and has the full set of genes encoding TCA cycle enzymes, but it is not clear which of these fumarate reductases play a role in these pathways. Strain SZ has five gene clusters (one on pSZ77) encoding pyruvate ferredoxin/flavodoxin oxidoreductase (PFOR) complexes (Additional file 7) of the type inferred to mediate incorporation of acetate into the TCA cycle in G. sulfurreducens. Strain SZ lacks any homologs to subunit A of the pyruvate dehydrogenase E1 complex, making it unclear how the organism is able to use pyruvate as an electron donor .
With regard to ROS and oxygen detoxification, strain SZ lacks homologs to the heme-copper (aa3) cytochrome c oxidases involved in the utilization of oxygen in G. sulfurreducens. Accordingly, strain SZ does not respire oxygen  and responds negatively to in situ oxygen exposure . Strain SZ possesses a complete gene cluster encoding homologs to the cytochrome bd ubiquinol oxidase complex shown to mediate oxygen tolerance in anaerobes (Glov_1208-Glov_1209, Additional file 8) , but lacks any homolog to the diheme cytochrome c peroxidase (MauG) or superoxide dismutase (SodA) shown to be strongly expressed in the presence of oxygen in G. uraniireducens. P. propionicus similarly lacks heme-copper (aa3) cytochrome c oxidase, MauG, and SodA. The absence of multi-heme c-type cytochromes with more than 12 hemes in strain SZ may reflect differences in habitat and metabolic strategy as compared to other Geobacter spp. The high-molecular weight c-type cytochromes with 13 or more hemes in G. sulfurreducens were suggested to function as biological capacitors , providing storage for reducing equivalents while the energy-stressed Geobacter cell is seeking new sources of terminal electron acceptors. Although this is an intriguing hypothesis, the synthesis of such high molecular weight c-type cytochromes under nutrient limitations burdens the cell’s energy budget, and the true benefit for survival has yet to be demonstrated. The poor oxygen tolerance exhibited by strain SZ, confining it to persistently anoxic habitats, may be associated with the reduced count of high molecular weight, multi-heme c-type cytochromes prevalent in aerotolerant Geobacter. Strain SZ may utilize a strategy for temporary electron storage that is not dependent on c-type cytochromes or is able to employ cytochromes with fewer hemes to the same effect seen in aerotolerant Geobacter.
Predicted heme and cobalamin biosynthesis genes on the G. lovleyi strain SZ genome in comparison to the model organism for anaerobic cobalamin biosynthesis, Salmonella enterica subsp. enterica ser. Typhimurium
G. lovleyi SZ genome
Homolog in Salmonella enterica subsp. enterica ser. Typhimurium LT2
% Ident. (%Simil.)
Early heme biosynthesis
δ-Aminolevulinic acid dehydratase
Late heme biosynthesis
Coproporphyrinogen III oxidase
Cobalamin biosynthesis protein
Cobalamin biosynthesis protein
Anaerobic cobalt chelatase
Cobyrinic acid a,c-diamide synthase
Siroheme synthase/ uroporphyrin-III C-methyltransferase
Cobalt ABC transporter, ATPase subunit
Cobalt ABC transporter, inner membrane subunit
Cobalt transport protein
Cobalt transport protein
Bifunctional adenosylcobinamide kinase /adenosylcobinamide-phosphate guanylyltransferase
Cobalamin biosynthesis protein
L-threonine O-3-phosphate decarboxylase/ cobyric acid synthase
Plasmid gene clusters associated with cobalamin metabolism
The strain SZ chromosome contains several genes encoding cobalamin-dependent enzymes including ribonucleotide reductase, methionine synthase, and methylmalonyl-CoA mutase; genes that are found in all other Geobacter genomes (Additional file 11). In addition, the strain SZ chromosome encodes two PCE RDases that presumably require a cobalamin co-factor . Accordingly, strain SZ has genes encoding all inferred functions necessary for de novo cobalamin biosynthesis plus a predicted outer membrane transport system, which may play a role in uptake of extracellular corrinoids. Unlike other Geobacter genomes, the majority of strain SZ cobalamin biosynthesis genes are encoded on the plasmid rather than the chromosome, localized in two gene clusters and a single gene locus (Table 4). The first cobalamin biosynthesis gene cluster comprises 15 consecutive or overlapping genes (Glov_3646 through Glov_3660) together encoding enzymes mediating the first 11 steps of de novo cobalamin biosynthesis and a cobalt ABC transporter. Genes 2 through 10 in the first cluster, cbiJ-H-G-F-D-L-K-C-A-CysG, along with one of the cobalt transport genes, cbiN, lack isofunctional homologs on the SZ chromosome suggesting the plasmid is essential when extracellular sources of cobalamin are lacking. The second cobalamin biosynthesis gene cluster comprises cobT (Glov_3678) on the reverse strand and cobD/cbiP (Glov_3679) on the leading strand, both showing evidence of duplication with genes on the SZ chromosome. CobT shares 78% aa identity with a chromosome-encoded CobT homolog. Similarly, the CobD/CbiP fusion shares 77% identity with a homologous fusion protein encoded on the SZ chromosome. The third locus is comprised of a single gene, cobA (Glov_3718), possibly involved in the adenosylation step of cobalamin biosynthesis. The pSZ77 CobA shares 36% aa identity with CobA encoded on the SZ chromosomal cobA locus. Genes encoding four of the final five steps of cobalamin biosynthesis, cbiB, and cobU cobS, and cobC, are found exclusively on the SZ chromosome (Table 4).
Nine genes highly similar to chromosomal orthologs are inferred in outer membrane transport and are situated between both cobalamin biosynthesis gene clusters on pSZ77 (Figure 3). Proteins encoded by genes at the 5’ and 3’ ends of this cluster, Glov_3667 and Glov_3675, were inferred by Pred-TMBB  to have up to 20 trans-membrane ß-strands characteristic of the TonB-dependent outer membrane receptors . Proteins encoded by Glov_3670, Glov_3669, and Glov_3668 have sequence characteristics matching the ExbB, ExbD, and TonB proteins involved in outer membrane transport of iron siderophores or vitamin B12[74, 75]. Glov_3671 encodes a protein annotated as a cobalt chelatase (CobN) (Figure 3) of the type involved in late cobalt insertion (i.e., “aerobic”) cobalamin biosynthesis . The confirmed CobN cobalt chelatase of Pseudomonas denitrificans is associated with pCobS and pCobT subunits . No genes predicted to encode homologs to these pCobS and pCobT accessory proteins were identified on the plasmid or chromosome of strain SZ, suggesting the pSZ77 CobN has a distinct function from its homolog in P. denitrificans. The pSZ77 putative CobN shared high aa similarity (79% identity) with a protein encoded by Gura_0774 on the G. uraniireducens chromosome, which is flanked by tonB receptor-related genes. These observations, coupled with its proximity to cobalamin biosynthesis clusters, suggest that the pSZ77-encoded CobN is associated with cobalamin metabolism in strain SZ, though its exact biochemical role is unknown. The cobN-exbB exbD tonB gene cluster matches chromosomal homologs in only two related genomes, G. uraniireducens and P. propionicus, and is predicted to function in the transport of iron siderophores, vitamin B12, or corrinoid precursors [74, 75, 78].
pSZ77 genes encoding cobalamin biosynthesis and cobalamin transport functions suggest that the plasmid might be essential for strain SZ growth. To examine the propensity for pSZ77 loss from G. lovleyi strain SZ, a series of protocols developed for curing non-essential plasmids from host organisms were assayed [19, 20]. SDS at concentrations above 0.1% inhibited growth of strain SZ, but growth was stable over 17 consecutive transfers with ≤0.018% SDS. L-ascorbate at concentrations of up to 10 mM did not affect growth over at least nine consecutive transfers. Strain SZ grew with PCE or fumarate as electron acceptor over at least 40 consecutive transfers in cyanocobalamin-free and cyanocobalamin-amended medium. In samples from all treatments that allowed growth, PCR with the repA-targeted primer pair yielded amplicons of the expected size suggesting that the plasmid carrying the repA DNA fragment was maintained under the growth conditions tested. The observation that strain SZ grew in medium with or without cyanocobalamin amendment indicated that the cobalamin biosynthesis genes on pSZ77 are functional and that pSZ77 is maintained under growth conditions that do not require cobalamin biosynthesis. Our efforts to cure strain SZ of pSZ77 were unsuccessful due either to pSZ77 genes being essential to SZ metabolism or due to the effectiveness of plasmid maintenance/stability-related genes. For example, the pSZ77 cobN-associated TonB-type outer-membrane transport genes, which match chromosomal orthologs in G. uraniireducens and P. propionicus, may have been critical to strain SZ metabolism in the vitamin B12-amended cultures. The pSZ77 tonB exbB exbD genes associated with cobN do not correspond to a complete cluster on the SZ chromosome, suggesting that transport of cobalamin and/or other organometallic complexes made the plasmid essential under the cultivation conditions used. With these apparently essential functions, pSZ77 shares one of the defining traits of the significantly larger secondary replicons observed in α- and β-Proteobacteria termed “chromids” . Alternatively, the pSZ77 genes predicted to contribute to plasmid stability, such as the partitioning ATPase (parB) or the toxin-antitoxin cluster (hipB-hipA) may be effective at maintaining the plasmid.
Origins of pSZ77 genes and horizontal gene transfer
Sequence analyses of pSZ77 genes involved in plasmid maintenance or recombination, cobalamin biosynthesis, and amino acid biosynthesis suggested that a significant portion of the plasmid originated from outside the Geobacteraceae. The protein encoded by the putative hipA (Glov_3688) shares 75% aa identity with a homolog encoded on the large plasmid (pPRO1) of P. propionicus. The pSZ77 replication protein, RepA, shows only distant phylogenetic relatedness with its Geobacter and Pelobacter homologs (Figure 5) and the repA locus has an atypical codon usage (normalized CAI < 1.00, Additional file 12) for both the SZ chromosome and plasmid. The pSZ77 RepA protein is most similar to homologs of γ-Proteobacteria (Figure 5), such as the RepA encoded on plasmid pBI1063 (36% aa identity, 56% aa similarity) from Stentrophomonas maltophilia. The predicted plasmid-partitioning gene, parA, lacks Geobacteraceae or Pelobacteraceae homologs altogether (Additional file 10). Additionally, Glov_3721, encoding a predicted vitamin B12-independent methionine synthase (MetE), lacks homologs in the Geobacteraceae and is functionally redundant to the metH locus on the SZ chromosome (Additional file 11).
The pSZ77 cobalamin biosynthesis genes correspond to isofunctional homologs on the chromosomes of all Geobacter spp., but gene fusions and gene order within the cobalamin biosynthesis clusters on pSZ77 are uncharacteristic of cobalamin biosynthesis gene clusters in any other Geobacter genome. For instance, the gene fusions sirA/cysG in the first cobalamin biosynthesis cluster and cobD/cbiP in the second cluster fully align with their orthologs on the Pelobacter carbinolicus chromosome, but share similarity with unfused genes encoded at distinct ORFs in other Geobacter genomes. The proteins encoded by genes of the first pSZ77 cobalamin biosynthesis cluster share an average of 56% aa sequence identity with their orthologs on the P. carbinolicus chromosome, higher than the average pairwise identity (46%) between the proteomes of strain SZ and P. carbinolicus. However, most of the genes in the first and second cobalamin biosynthesis clusters have normalized CAIs > 1.00 (Additional file 12) suggesting the portion of pSZ77 encoding the cobalamin biosynthesis genes have resided in the SZ genome for sufficient time to ameliorate to its average codon usage [29, 53].
Distribution of SZ-like plasmids among dechlorinating Geobacter strains
A G. lovleyi strain sharing 99% 16S rRNA gene identity with strain SZ was isolated from the PCE-to-ethene-dechlorinating consortium KB-1 in order to examine its plasmid complement. This Geobacter isolate was found to be capable of dechlorinating PCE to cis-DCE, and was designated G. lovleyi strain KB-1. Metagenome sequencing of the KB-1 consortium and plasmid isolation and sequencing from the Geobacter strain KB-1 isolate revealed that strain KB-1 carries a 77,215 bp plasmid sharing high similarity with pSZ77 (99% identity). Although they were originally isolated from different locations and habitats (i.e., strain SZ from non-contaminated Su-Zi Creek freshwater sediment, South Korea , and strain KB-1 from a chlorinated solvent-contaminated aquifer in Ontario, Canada ), the PCE-to-cis-DCE dechlorinating G. lovleyi strain KB-1 and strain SZ share highly similar plasmids. The sequence differences between pSZ77 and the KB-1 assembly were comprised mainly of short insertions and deletions. An intact ORF encoding a recombinase on the KB-1 plasmid assembly corresponds to a pseudogene (Glov_3699) with a 119 bp deletion on pSZ77. A 136 bp insertion occurs on pSZ77 of strain SZ in an intergenic region upstream of Glov_3713 encoding a transposase. There are six apparent deletions in the KB-1 assembly relative to the pSZ77 DNA sequence, ranging in length from 1 bp to 136 bp (Additional file 13), but the majority of these deletions occur in positions in the assembly with two-fold sequence coverage or less, and so cannot be considered reliable. Out of the 79 intact ORFs shared between pSZ77 and the KB-1 assembly, only two single nucleotide polymorphisms could be found. Apparently, the pSZ77 and the KB-1 plasmid assemblies exhibit few sequence differences and little difference in gene repertoire. Likewise, the strain SZ genome shares ~99% identity with metagenome contigs obtained from consortium KB-1. Based on the geographically- and ecologically-disparate habitats of strain SZ and strain KB-1, the strictly anaerobic, non-spore-forming species G. lovleyi appears to be cosmopolitan.
The identification of the plasmid from strain KB-1 suggested that the 77 kbp plasmid is a shared feature of members of the organohalide-respiring Geobacter clade. To test this hypothesis, primers targeting the repA plasmid maintenance gene were used to amplify the pSZ77 repA gene from members of the organohalide-respiring Geobacter clade. Included in the analysis were four additional G. lovleyi isolates obtained from the chlorinated ethene-contaminated site, Fort Lewis, whose 16S rRNA gene sequences suggest a close phylogenetic relationship (Additional file 1) with strain SZ (99–100% nucleotide identity). Amplicons from the repA-targeted primers of the expected size and sequence were obtained with template DNA from strain SZ (positive control), strain KB-1 (not shown), and the BDI and KB-1 consortia (Additional file 14). No amplicons were obtained from any of the other organohalide-respiring Geobacter isolates, suggesting that the Fort Lewis soil G. lovleyi isolates Geo7.1, Geo7.2, Geo 7.3, and Geo7.4 do not contain a pSZ77-like plasmid. The primer set Geo564F/840R  was successfully used to amplify Geobacteraceae 16S rRNA genes from all samples. To further verify that the Fort Lewis Geobacter isolates lack plasmids, plasmid extractions were performed with biomass from the Fort Lewis G. lovleyi isolates, G. thiogenes, and G. lovleyi strain SZ. Only strain SZ DNA yielded the characteristic 77 kbp plasmid, and no bands indicative of a plasmid were observed in the other samples (data not shown). The lack of physical DNA in plasmid DNA extractions from G. thiogenes and the Fort Lewis G. lovleyi strains were consistent with the repA-targeted PCR results, suggesting neither G. thiogenes nor the Fort Lewis strains contain a similar plasmid. The lack of evidence for pSZ77-like plasmids in several PCE-respiring Geobacter suggested that pSZ77 does not play a direct or obligate role in organohalide respiration. Mechanisms for cobalamin biosynthesis or transport of corrinoids are essential for respiration with PCE and other chlorinated ethenes [44, 80]. This implies that the Geobacter strains without a plasmid likely either have chromosome-encoded cobalamin biosynthesis genes or an efficient mechanism for uptake of extracellular corrinoids. While not indispensable to reductive dehalogenation across all G. lovleyi strains, pSZ77 may play an indirect adaptive role in PCE respiration in strains SZ and KB-1. For instance, pSZ77 may be important to strains SZ and KB-1 by allowing increased gene copy numbers or differential regulation of gene functions relevant for organohalide respiration . The two cobalamin biosynthesis clusters on pSZ77 situated on either side of the TonB-encoding transport cluster (Figure 3) may thus provide strains SZ and KB-1 with a mechanism for simultaneous regulation of both cobalamin biosynthesis and scavenging for extracellular corrinoids.
Phylogenetic analysis and sequence features related to respiratory metabolism support the affiliation of strain SZ within the Geobacter genus. Compared to other Geobacter genomes, strain SZ carries an unusually large quantity of laterally acquired DNA including the genomic island with duplicated pceAB gene clusters enabling strain SZ to respire PCE and TCE. The origin of the pce-genes is unclear given that they share low similarities with their top BlastP matches in Desulfitobacterium spp. The frequency and mechanism of transfer of the Pce genomic island are not yet understood, and as such the consequences of potential lateral transfer to non-dechlorinating Geobacter spp. are unclear; however, such events are obviously relevant for bioremediation. Up to 69% of strain SZ genes shared their top BlastP matches with other Geobacter spp. or Pelobacter spp. genomes (Table 1). At the same time, 9% of strain SZ genes did not have homologs in the sequenced representatives of the δ-Proteobacteria phylum. Notably, the SZ conjugative pilus tra-gene cluster comprises a portion of the largest chromosomal genomic island yet described in a Geobacter genome. The tra-genes may provide strain SZ with a mechanism for enhanced uptake of foreign DNA. Compared to other Geobacter genomes, strain SZ possesses fewer c-type cytochrome genes with no more than 12 heme binding motifs, which does not appear to limit the organism’s ability to respire oxidized metal species.
A distinguishing feature of the G. lovleyi genome is pSZ77, an extrachromosomal element carrying genes that are typically located on the chromosome in other Geobacteraceae. The phylogenetic origins of the pSZ77 repA and parA genes are unclear and may have been laterally acquired separately from the pSZ77 genes with chromosomal orthologs. Most notable among its genes with predominantly chromosomal homologs, pSZ77 encodes 15 out of the 24 genes required for de novo cobalamin biosynthesis. Although a nearly identical plasmid occurs in G. lovleyi strain KB-1, not all organohalide-respiring Geobacter lovleyi strains carry a non-chromosomal element, indicating that pSZ77 is not a marker for PCE reductive dechlorination within this species. On the other hand, unique genes and gene clusters, particularly the pce-genes, may provide discriminative detection of PCE-respiring Geobacter strains.
We thank the DOE Joint Genome Institute for sequencing the genome of G. lovleyi strain SZ. This research was supported by the Strategic Environmental Research and Development Program (SERDP) under contract W912HQ-10-C-0062 (project ER-1586), the U.S. DOE Office of Science (OBER), Subsurface Biogeochemical Research (SBR) Program (grant no. ER64782), and the Government of Canada through Genome Canada and the Ontario Genomics Institute (2009-OGI-ABC-1405).
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