Centromere-associated repeat arrays on Trypanosoma brucei chromosomes are much more extensive than predicted

BMC Genomics

Table 1 Inferred sizes of the centromeric tandem arrays on chromosomes 1 - 8.

Chr no.	Chr size	Size of centromeric repeat array (GeneDB)	Size inferred^a from mapping	Artemis coordinates^b
1	1.15/1.2 Mb	5.2 kb	20 & 65 kb	780179....785416
2	1.3 Mb	8.2 kb	30 & 55 kb	290326....298494
3	1.8/2.0 Mb	7.8 kb	75 & 80 kb	884223....891983
4	1.9/2.0 Mb	3.6 kb	70 kb	954159....957772
5	2.0 Mb	1.9 kb	50 & 75 kb	197919....199815
6	2.0/2.5 Mb	5.4 kb	55 kb	59066....64433
7	2.7 Mb	3.1 kb	100 & 120 kb	1936979....1940105
8	2.7/2.9 Mb	unknown^c	100 kb	2233201....2233419^d

^aThe approaches used to infer the extent of each of the centromeric repeat arrays are outlined in the text (chromosomes 3, 5 and 7) and Additional file 2 (chromosomes 1, 2, 4, 6 and 8).
^bThe coordinates were defined using the Tandem Repeats Finder Program [31]. The positions in the sequence where the programme detected the first and the last motif were defined as the beginning and as the end of the centromeric repeat region. Coordinates are from T. brucei 927 version 4 (GeneDB).
^cThe size of the centromeric repeat array is not defined in GeneDB due to incomplete gap closure.
^dThe coordinates correspond to blast hits with chromosome 4 centromeric repeat sequences. These occur adjacent to a gap of undetermined size.

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