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Table 7 The primer sequences used for SYBR Green RT-PCR

From: Microarray analysis on germfree mice elucidates the primary target of a traditional Japanese medicine juzentaihoto: acceleration of IFN-α response via affecting the ISGF3-IRF7 signaling cascade

Specificty

Primer pair

Product size (bp)

Stat 1

5'-CACGCTGCCTATGATGTCTC-3'

5'-ACGCTTGCTTTTCCGTATGT-3'

132

Stat2

5'-CTTGTTCTTGGGTGGAGCACC-3'

5'-TTGGTGTAGGGCTGCACTGAG-3'

67

Isgf3g

5'-CATAGTTGGCACATGTGAGACA-3'

5'-ATCTCTCCAGCCGCTCTTAG-3'

121

Irf7

5'-CCCATCTTCGACTTCAGCAC-3'

5'-TGTAGTGTGGTGACCCTTGC-3'

88

Ifn-α

5'-GAATGCAACCCTCCTAGAC-3'

5'-GTCAGAGGAGGTTCCTG-3'

105

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BMC Genomics

ISSN: 1471-2164

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