Figure 4From: Characterization of microRNAs expression during maize seed developmentNovel miRNAs validated by stem-loop RT-PCR. Four novel miRNAs (miR03, miR23 and miR34 from seed, miR19 from leaf) were validated by RT-PCR and sequencing. 30 cycles of standard PCR was used to amplify Actin. A non-specific band was found in the miR34 PCR product (upper).Back to article page