Figure 1

Marker map of the genomic region around  Rhg1/Rfs2  and the most identical paralog (homeolog) of  Rhg1/Rfs2  with locus, BAC and gene ideograms. Panel A shows the marker map of the genomic region around Rhg1/Rfs2 (Lg G; chromosome 18) with locus ideograms. Sequence coordinates were from the susceptible cultivar Asgrow 3244 [16, 49]. The GmRLK18-1 gene encoding the RLK was shown as a black block arrow. The genes encoding the laccase and antiporter were shown as opposite white block arrows. All other genes were shown as grey block arrows. Locations of overlapping BAC clones B73p06 and B21d09 that both encoded the GmRLK18-1 at the Rhg1/Rfs2 locus (Lg G; chromosome 18) were shown below the ideogram. Panel B shows the BAC clone B73p06 that encoded the Rhg1/Rfs2-a locus. The gene encoding the RLK was shown as a black block arrow. The genes encoding the laccase and antiporter were shown as opposite white block arrows. All other genes were shown as grey block arrows. The extent of the pSBHB94 (HQ008939), the 9.772 kbp subclone from BAC B21d09 that was used for soybean transformations was shown as a blue arrow. The plasmid pSBHB94 encompassed from 30,423- 40–194 bp. Sequence coordinates were from the complete sequence of the BAC derived from resistant cultivar Forrest (HQ008938). Panel C showed a syntenic homeolog of Rhg1-a /Rfs2/ found in the sequence of BAC H38F23 from Lg B1 (chromosome 11). The homeolog of the gene encoding the RLK was show as a black block arrow. The homeologs of the genes encoding the laccase and antiporter were shown as opposite white block arrows. All other syntenic genes were shown as grey block arrows. The marker TMD1 amplified a fragment from Rhg1-a /Rfs2/Rhg1-a/Rfs2 of 303+ 15 bp (resistant allele was the smaller) and of 362 bp from a syntenic homeolog of Rhg1-a /Rfs2Rfs2/ found in the sequence of BAC H38f23 from Lg B1 (chromosome 11). Sequence coordinates were from the complete sequence of the BAC derived from resistant cultivar Forrest (HQ008940). Panel D shows an ideogram of markers, the GmRLK18-1 gene and gene features found in the insert of plasmid pSBHB94 that encompassed from 30,423- 40,194 bp of B73p06. Panel E shows the position of markers in the transcribed region of the GmRLK18-1 gene. Panel F shows the position of amino acid substitutions in the protein encoded by the cDNA.