Figure 2From: Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicidaMonitoring Spot 42 and VSsrna24 expression under different treatments. (A) A. salmonicida was cultured for approximately 30 hours (h) starting at OD600 nm 0.1, and ending at 1.8 (stationary phase). A typical growth trajectory of A. salmonicida in LB is illustrated in the figure. Samples were collected throughout the growth cycle. The culture was split and 1 mM cAMP was added at OD600 nm 0.4 to one half. Similarly, a second culture was split and 5 mM glucose was added at OD600 nm 1.2. Untreated cells were used as control. (B) Samples collected in A were subjected to Northern blot analysis. From the control culture samples were collected at OD600 nm 0.2-1.7, and after the additions of cAMP or glucose samples were collected after 5-240 min (OD600 nm 0.4-0.8) or 5-360 (OD600 nm 1.2-1.5) min, respectively. Radio-labeled double-stranded DNA probes were used to monitor the levels of Spot 42 and VSsrna24, and 5S rRNA was used to normalize the result. Fold change values ≥ 2 are shown below samples, and are always comparisons between treated and untreated control samples, at same ODs.Back to article page