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Figure 2 | BMC Genomics

Figure 2

From: Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida

Figure 2

Monitoring Spot 42 and VSsrna24 expression under different treatments. (A) A. salmonicida was cultured for approximately 30 hours (h) starting at OD600 nm 0.1, and ending at 1.8 (stationary phase). A typical growth trajectory of A. salmonicida in LB is illustrated in the figure. Samples were collected throughout the growth cycle. The culture was split and 1 mM cAMP was added at OD600 nm 0.4 to one half. Similarly, a second culture was split and 5 mM glucose was added at OD600 nm 1.2. Untreated cells were used as control. (B) Samples collected in A were subjected to Northern blot analysis. From the control culture samples were collected at OD600 nm 0.2-1.7, and after the additions of cAMP or glucose samples were collected after 5-240 min (OD600 nm 0.4-0.8) or 5-360 (OD600 nm 1.2-1.5) min, respectively. Radio-labeled double-stranded DNA probes were used to monitor the levels of Spot 42 and VSsrna24, and 5S rRNA was used to normalize the result. Fold change values ≥ 2 are shown below samples, and are always comparisons between treated and untreated control samples, at same ODs.

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