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Table 2 Read metrics for both sequencing runs described

From: Ultra-high resolution HLA genotyping and allele discovery by highly multiplexed cDNA amplicon pyrosequencing

 

Run 1

Run 2

Raw Wells

194,989

220,452

Keypass Wells

193,002

218,741

Filterpass Wells

74,101

77,977

Ave Length (st. dev)

482 (108)

487 (91)

Samples Multiplexed

52

92

Amplicons Multiplexed

116

271

Reads per Sample (st. dev)

1,319 (520)

825 (504)

Amp 1 Reads per Sample (st.dev)

542 (167)

386 (142)

Amp 2 Reads per Sample (st. dev)

592 (272)

352 (247)

DRB Reads (st. dev)

803 (378)

92 (79)

  1. The number of raw, keypass, and filterpass reads per run are shown, along with the number of samples multiplexed, and the average read number for each amplicon included in the pool.