Figure 3
From: Computational prediction and validation of C/D, H/ACA and Eh_U3 snoRNAs of Entamoeba histolytica
Expression analysis of E. histolytica snoRNAs by northern blotting. 15 μg of total RNA enriched in small RNA was resolved on a 12% denaturing urea PAGE gel. For Eh-U3 snoRNA 10 μg of total RNA was electrophoresed on 1.2% denaturing agarose. Blots were transferred to nylon membrane and hybridized to P32 DNA probe specific to each snoRNA. Northern blot analysis of computationally-predicted C/D box (A) H/ACA box (B) snoRNAs. The 70 nt tRNA-Glu(AAA) of E. histolytica was used as a positive control, as indicated in the lower panel of selected samples. Table displaying the predicted (see Tables1 and2) and observed sizes of snoRNAs (C). Sizes of bands were marked by end labelled P32 decade marker (10 – 150 nt, Ambion).