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Table 1 Box C/D snoRNA genes in E. histolytica

From: Computational prediction and validation of C/D, H/ACA and Eh_U3 snoRNAs of Entamoeba histolytica

snoRNA genes

Len.

Seq.

Modification

Antisense element

Scaffold

Start

End

Location

(nt)

(%)

**Me-Eh-SSU-G1296

78

92%

SSU-G1296

12nt(5') 100%

DS571223

24176

24254

IR

   

SSU-G1298

10nt(5’) 100%

    
   

SSU-G1195

10nt(5’) 100%

    

Me-Eh-SSU-U1024

80

96%

SSU-U1024

14nt(5') 95%

DS571261

44605

44684

IR

   

SSU-U1822

11nt(5’) 98%

    

**Me-Eh-SSU-A83

78

100%

SSU-A83

16nt(5') 100%

DS571196

58225

58327

IR

   

SSU-U87

12nt(5’) 100%

    

Me-Eh-SSU-G41

68

93%

SSU-G41

11nt(5’) 100%

DS571147

177417

177350

IR

Me-Eh-SSU-A431

68

94%

SSU-A431

13nt(5') 100%

DS571331

10236

10303

IR

Me-Eh-SSU-U871

80

96%

SSU-U871

20nt(5’) 95%

DS571673

2402

2481

NA

*Me-Eh-SSU-G1535

82

93%

SSU-G1535

12nt(5') 100%

DS571215

31121

31040

IR

   

LSU-G2053

9nt(5') 100%

    

Me-Eh-SSU-A27

66

100%

SSU-A27

11nt(5') 100%

DS571226

26372

26307

IR

Me-Eh-SSU-A1830

83

88%

SSU-A1830

11nt(5') 100%

DS571152

29351

29433

EHI_049420 (+)

Me-Eh-SSU-A836

103

--

SSU-A836

13nt(5’)

DS571152

99242

99140

IR

Me-Eh-SSU-G1152

60

91%

SSU-G1152

12nt(3') 100%

DS571335

19522

19581

IR

Me-Eh-SSU-G628

97

--

SSU-G628

10nt(5’)

DS571451

15436

15532

IR

     

DS571177

52928

52831

 

Me-Eh-SSU-A1183

82

--

SSU-A1183

10nt(5’)

DS571164

22795

22876

IR

   

SSU-G1836

13nt(5’)

    
   

SSU-A1485

9nt(5’)

    
   

LSU-A520

12nt(3’)

    
   

LSU-U1210

12nt(5’)

    
   

LSU-A145

10nt(3’)

    

Me-Eh-SSU-A790

68

94%

SSU-A790

10nt(5’) 100%

DS571171

51701

51634

IR

   

SSU-A1496

11nt(5’) 100%

    
   

LSU-A801

11nt(5’) 100%

    
   

LSU-A1834

10nt(5’) 100%

    
   

LSU-A2555

11nt(5’) 100%

    

Me-Eh-SSU-C1805

63

96%

SSU-C1805

10nt(5') 100%

DS571145

496851

496789

IR

Me-Eh-LSU-A928a

69

97%

LSU-A928

11nt(5') 100%

DS571323

13072

13140

IR

Me-Eh-LSU-A928b

66

98%

LSU-A782

11nt(5') 100%

DS571163

50734

50669

IR

   

LSU-A928

9nt(5’) 100%

    
   

LSU-A1034

9nt(5’) 100%

    

Me-Eh-LSU-U1868

101

92%

LSU-U1868

13nt(5’) 92.3%

DS571175

28933

28833

IR

Me-Eh-LSU-U3580a

103

--

LSU-U3580

19nt(5’)

DS571304

677

575

IR

Me-Eh-LSU-U3580b

105

--

LSU-U3580

19nt(5’)

DS571305

36390

36494

IR

Me-Eh-LSU-A785

62

96%

LSU-A785

13nt(5') 100%

DS571416

15678

15739

IR

Me-Eh-LSU-G2958

70

97%

LSU-G2958

13nt(5') 100%

DS571205

22350

22419

IR

*Me-Eh-LSU-A3089

71

92%

LSU-A3089

11nt(5’) 100%

DS571180

41005

40935

IR

*Me-Eh-LSU-C2414

69

97%

LSU-C2414

11nt(5') 100%

DS571473

957

1025

IR

Me-Eh-LSU-G926

59

98%

LSU-G926

13nt(3') 100%

DS571150

13447

13389

IR

Me-Eh-LSU-U1018

69

--

LSU-U1018

11nt(5’)

DS571215

62034

62102

IR

   

LSU-U2783

14nt(5’)

DS571316

3067

2999

 

Me-Eh-LSU-G1028

61

87%

LSU-G1028

14nt(3’) 100%

DS571174

92482

92422

IR

Me-Eh-LSU-U1176a

109

94%

LSU-U1176

14nt(5') 100%

DS571307

17712

17820

IR ▲

Me-Eh-LSU-U1176b

109

94%

LSU-U1176

14nt(5') 100%

DS571419

10643

10535

IR

Me-Eh-LSU-U1176c

109

93%

LSU-U1176

14nt(5') 100%

DS571792

3710

3820

IR

Me-Eh-LSU-A2333

128

93%

LSU-A2333

12nt(3') 100%

DS571208

15564

15691

IR

**Me-Eh-LSU-A228

72

97%

LSU-A228

13nt(5') 100%

DS571397

17920

17991

EHI_003940

Intron of gene

40 S ribosomal protein S4, putative

**Me-Eh-5.8 S-U84

62

86%

5.8 S-U84

18nt(3’) 91%

DS571194

27534

27595

3’UTR

*Me-Eh-5.8 S-A92

115

85%

5.8 S-A92

11nt(5’) 94%

DS571180

76405

76291

EHI_118830 (−) ■

  1. ** snoRNAs validated by RT-PCR and Northern, * validated only by RT-PCR.
  2. Note: “Len.” denotes length of the snoRNA genes, “Seq.” is sequence identity of corresponding snoRNA genes in E. dispar, “Antisense element” denotes length of antisense element in E. histolytica and its sequence identity with E. dispar. “IR”, intergenic region, “NA”, no annotation. snoRNA located close to ribosomal protein genes , downstream to rRNA methyltransferase gene ▲, close to C/D box snoRNP (fibrillarin) ■. (+) and (−) represents snoRNA in sense and antisense orientation with respect to host gene.