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Table 2 Box H/ACA snoRNA genes in E. histolytica

From: Computational prediction and validation of C/D, H/ACA and Eh_U3 snoRNAs of Entamoeba histolytica

snoRNA genes

Len

Seq

Modification

Antisense element

Scaffold

Start

End

Location

(nt)

(%)

**ACA-Eh-SSU1315

121

96%

SSU1315

6 + 7nt (5’) 100%

DS571149

98793

98673

IR

ACA-Eh-SSU631

137

-

SSU631

6 + 5nt (3’)

DS572405

485

349

NA

  

-

SSU1114

8 + 9nt (5’)

DS572405

485

349

 

ACA-Eh-SSU1727

135

87%

SSU1727

9 + 5nt (5’) 93%

DS571346

12499

12633

IR/5'UTR

**ACA-Eh-SSU626

127

94%

SSU626

6 + 6nt(3') 100%

DS571463

13091

12965

IR

ACA-Eh-SSU461

142

-

SSU461

7 + 5nt (3’)

DS571171

90117

90258

IR

ACA-Eh-SSU1675

127

92%

SSU1675

5 + 9nt (3') 93%

DS571182

71521

71647

IR

*ACA-Eh-SSU526

126

94%

SSU526

7 + 5nt (5') 100%

DS571463

12972

13097

IR

*ACA-Eh-LSU3008

129

92%

LSU3008

7 + 5nt (5') 100%

DS571272

39423

39295

IR

ACA-Eh-LSU1172a

142

-

LSU1172

5 + 4nt (5’)

DS571149

73439

73580

IR

ACA-Eh-LSU1172b

141

-

LSU1172

5 + 4nt (3’)

DS571307

22719

22859

IR

ACA-Eh-LSU1107b

155

-

LSU1107

11 + 3nt (5’)

DS571159

2240

2086

IR

  

-

LSU1172

6 + 8nt (3’)

DS571159

2240

2086

 
  

-

5.8 S52

8 + 3nt (3’)

DS571159

2240

2086

 

ACA-Eh-LSU1650

118

89%

LSU1650

8 + 5nt (5’) 100%

DS571267

21025

21142

IR

ACA-Eh-LSU3087

129

92%

LSU3087

6 + 4nt (5') 100%

DS571178

75373

75501

IR

ACA-EH-LSU2791

161

 

LSU2791

6 + 7nt (5’)

DS571159

59530

59690

IR

ACA-Eh-LSU3155

151

88%

LSU3155

5 + 6nt (3') 91%

DS571255

1114

963

IR

ACA-Eh-LSU3221

152

79%

LSU3221

9 + 4nt (5’) 91.6

DS571339

14712

14561

IR

ACA-Eh-LSU1159a

154

-

LSU1159

4 + 5nt (5’)

DS571589

7973

8126

IR

     

DS571660

2209

2056

 

ACA-Eh-LSU2700

144

86%

LSU2700

8 + 3nt (3') 100%

DS571160

113417

113560

IR

 

144

 

LSU1159

6 + 4nt (3') 100%

DS571160

113417

113560

 

ACA-Eh-LSU1080

123

-

LSU1080

3 + 7nt (5’)

DS571228

4519

4641

IR

**ACA-Eh-LSU1343

137

-

LSU1343

5 + 5nt (5’)

DS571219

12011

11875

IR

ACA-Eh-LSU2997b

129

96%

LSU2997

5 + 4nt (5') 100%

DS571145

384477

384605

IR

ACA-Eh-LSU339

148

-

LSU339

5 + 4nt (5’)

DS571174

50939

50792

IR

ACA-Eh-LSU1123

148

-

LSU1123

4 + 7nt (5’)

DS571225

51991

52138

IR

ACA-Eh-LSU1005

148

-

LSU1005

4 + 5nt (3’)

DS571402

1263

1116

IR

ACA-Eh-LSU1236a

141

-

LSU1236

3 + 6nt (3’)

DS571481

789

649

IR

ACA-Eh-LSU1236b

141

-

LSU1236

3 + 6nt (3’)

DS571159

21643

21503

IR

ACA-Eh-LSU1107a

154

-

LSU1107

11 + 4nt (3’)

DS571208

46788

46941

IR/ 5'UTR

  

-

SSU1114

8 + 9nt (5’)

DS571208

46788

46941

 

**ACA-Eh-LSU2288

126

92%

LSU2288

4 + 9nt(5') 100%

DS571148

172182

172057

IR

   

SSU1431

6 + 4nt (3') 90.0%

DS571148

172182

172057

 

ACA-Eh-LSU1159b

153

-

LSU1159

5 + 5nt (5’)

DS572251

153

1

NA

  

-

LSU3221

4 + 6nt (3’)

DS572251

153

1

 
  

-

SSU826

4 + 6nt (5’)

DS572251

153

1

 

ACA-Eh-LSU2997a

122

-

LSU2997

5 + 6nt (5’)

DS572347

1128

1007

NA

     

DS572347

800

679

 
     

DS572347

464

343

 
     

DS572347

132

11

 

ACA-Eh-5.8 S80a

140

-

5.8 S80

5 + 9nt (5’)

DS571346

5092

4953

IR

ACA-Eh-5.8 S80b

132

-

5.8S80

5 + 6nt (5’)

DS571206

1568

1437

IR

  

-

LSU3221

5 + 5nt (5’)

DS571206

1568

1437

 

ACA-Eh-SSU740

141

92%

SSU740

4 + 7nt (3') 91%

DS571156

54460

54320

EHI_182810 (+)

*ACA-Eh-SSU188

135

93%

SSU188

6 + 3nt (5’) 89%

DS571501

5129

5263

EHI_172000 (+)

*ACA-Eh-SSU1216

142

77%

SSU1216

5 + 4nt (3’) 89%

DS571247

8141

8000

EHI_016340 (−)

ACA-Eh-SSU299

169

94%

SSU299

4 + 6nt (3') 100%

DS571161

119527

119695

EHI_142230 (+)

ACA-Eh-SSU1212

129

93%

SSU1212

9 + 7nt (3') 100%

DS571169

105772

105900

EHI_098580 (−)

**ACA-Eh-LSU2809

156

82%

LSU2809

12 + 3nt(3') 86.7%

DS571148

116513

116668

EHI_012330 (−)

ACA-Eh-LSU2335

131

93%

LSU2335

3 + 6nt (5’) 100%

DS571304

17766

17896

EHI_161910 (−)

ACA-Eh-LSU2493

135

87%

LSU2493

8 + 3nt (5’) 82%

DS571228

40854

40720

EHI_161000 (−)

ACA-Eh-LSU1176

157

97%

LSU1176

5 + 4nt (5') 100%

DS571185

32437

32593

EHI_104450 (+)

ACA-Eh-LSU2268

135

97%

LSU2268

7 + 3nt (3') 90%

DS571154

24191

24057

EHI_178500 (−)

*ACA-Eh-5.8 S84

152

82%

5.8 S84

7 + 5nt (5’) 92%

DS571169

105495

105646

EHI_098580 (−)

  1. ** snoRNAs validated by RT-PCR and Northern, * validated only by RT-PCR.
  2. Note: “Len.” denotes length of the snoRNA genes, “Seq.” is sequence identity of corresponding snoRNA genes in E. dispar, “Antisense element” denotes length of antisense element in E. histolytica and its sequence identity with E. dispar. “IR”, intergenic region, “NA”, no annotation. snoRNA located close to ribosomal protein genes . (+) and (−) represents snoRNA in sense and antisense orientation with respect to host gene.