Table 4 Statistics of reads mapped to assemblies
From: Optimizing de novo common wheat transcriptome assembly using short-read RNA-Seq data
Proportion of reads | Trinity | Trans-ABySS | ||||
|---|---|---|---|---|---|---|
|  | AFSFASSS | AFSF + ASSS | AF + SF + AS + SS | AFSFASSS | AFSF + ASSS | AF + SF + AS + SS |
AF aligned | 83.83% | 87.53% | 89.17% | 93.62% | 92.44% | 91.48% |
  aligned more than 3 hits | 4.18% | 8.12% | 9.63% | 2.93% | 3.89% | 4.91% |
  uniquely aligned | 51.85% | 48.67% | 48.48% | 71.74% | 67.46% | 63.89% |
AS aligned | 82.21% | 86.50% | 88.27% | 92.82% | 91.85% | 90.73% |
  aligned more than 3 hits | 4.23% | 8.35% | 10.45% | 3.00% | 3.85% | 4.93% |
  uniquely aligned | 50.67% | 48.48% | 47.70% | 70.94% | 66.89% | 62.74% |
SF aligned | 80.06% | 86.13% | 88.61% | 93.55% | 92.23% | 91.41% |
  aligned more than 3 hits | 3.88% | 8.30% | 11.46% | 3.92% | 4.79% | 6.08% |
  uniquely aligned | 50.48% | 48.73% | 47.06% | 71.63% | 67.09% | 62.58% |
SS aligned | 74.98% | 86.39% | 88.47% | 92.67% | 91.68% | 90.81% |
  aligned more than 3 hits | 3.81% | 7.83% | 10.71% | 3.19% | 3.98% | 5.07% |
  uniquely aligned | 46.73% | 51.45% | 49.93% | 72.35% | 68.42% | 64.23% |
All aligned | 79.89% | 86.57% | 88.52% | 93.01% | 91.95% | 90.98% |
  aligned more than 3 hits | 4.04% | 8.15% | 10.56% | 3.19% | 4.04% | 5.15% |
  uniquely aligned | 49.60% | 49.47% | 48.41% | 71.61% | 67.48% | 63.35% |