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Table 5 Comparisons between full-length cDNA transcripts and assembled transcripts

From: Optimizing de novo common wheat transcriptome assembly using short-read RNA-Seq data

6,137 fl-cDNA transcripts

Trinity

Trans-ABySS

 

AFSFASSS

AFSF + ASSS

AF + SF + AS + SS

AFSFASSS

AFSF + ASSS

AF + SF + AS + SS

% of fl-cDNA hit

92.94%

92.83%

92.62%

91.33%

90.39%

89.69%

(% of bases covered)

82.84%

82.64%

81.68%

79.52%

76.71%

74.09%

% of fl-cDNA hit with at least 90% of its length

92.06%

92.05%

92.13%

90.83%

89.73%

88.90%

(% of bases covered)

82.08%

82.02%

81.14%

79.24%

76.40%

73.79%