Figure 1

Schematic diagram of PPO domains and conserved residues. (A) Typical PPOs contain an N-terminal transit peptide (green), which is cleaved at an alanine motif (inverted triangle) after import into the thylakoid lumen. The conserved CuA and CuB domains are shown in blue, the C-terminal domains in grey. (B) WebLogo sequence logos indicating conserved residues in PPO domains. The first 35 amino acids of the transit peptide are shown (underlined in grey). The thylakoid transfer domain, the alanine (AxA) cleavage motif, the DWL motif, the tyrosine (YxY) motif and the KFDV motif are underlined in black. The three conserved histidine residues in both the CuA and CuB domains are numbered and shown in blue. Black stars indicate absolutely conserved residues. The boxed sequences in the PPO1_KFDV domain are conserved regions identified in this study.