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Figure 2 | BMC Genomics

Figure 2

From: High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

Figure 2

CcpA expression levels in B. subtilis cultures used for DNA microarray experiments. (A) upper panel, CcpA detection using anti-CcpA antibody; lower panel, signal quantification with ImageJ. Four CcpA expression levels were achieved by growing B. subtilis strain MP902 (Ptet-ccpA, Pxyl-tetR) in absence (lanes 1, 3 and 5) and in presence of 0.1, 2 and 20 nM ATc (lanes 2, 4 and 6), respectively. All cultures were grown in presence of 0.2 % xylose and 1 % glucose. Shadows in the background of the picture indicate culture pairs used in microarray experiments (B) Ponceau S control membrane staining for protein load verification. Lane numbers correspond to lane numbers in panel A. The representative graphs of three reproducible experiments are shown.

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