Hepatic transcriptome analysis of inter-family variability in flesh n-3 long-chain polyunsaturated fatty acid content in Atlantic salmon

Table 6 Validation of microarray results and expression of genes of interest in relation to the factor 'Lipid level'

Gene	Low n-3 LC-PUFA HL/LL		High n-3 LC-PUFA HH/LH
	Microarray	RT-qPCR	Microarray	RT-qPCR
LC-PUFA biosynthesis
Δ5fad		−1.03		1.35
Δ6fad		1.04		−1.32
elovl2	−1.28 to −1.41	−1.51	−1.82 to −1.97	−1.62
Cholesterol biosynthesis
ipi		−3.95		1.31
mev		−1.40		1.14
7dchr		−1.01		1.09
srebp2		−2.09		1.29
Lipid transport and lipoprotein metabolism
lrp1		−1.82		−1.08
apoA4a		−1.37		−0.95
apoA4b		−2.43		−1.80
lpl		−1.18		−1.05
Immune response
cfh	−1.33 to −1.56	−1.24	−1.56 to −1.69	−1.31
trim25	−5.64	−2.09	−4.34	−2.33
lect2	3.16	1.23	4.86	4.84

Values represent the expression ratios between high lipid / low lipid, for fish containing either low or high n-3 LC-PUFA levels in their flesh, obtained by microarray analysis or RT-qPCR. Expression ratios in bold were significant by REST2008 analysis of RT-qPCR results.
Delta5 and 6 fatty acyl desaturases (Δ5fad and Δ6fad); fatty acyl elongase (elovl2); isopentenyl-diphosphate isomerase (ipi); mevalonate kinase (mev); 7-dehydrocholesterol reductase (7dchr); sterol regulatory element-binding protein 2 (srebp2); low density lipoprotein receptor-related protein 1 (lrp1); apolipoprotein A-IV (apoa4a and apoa4b); lipoprotein lipase (lpl); complement factor H precursor (cfh); tripartite motif-containing protein 25 (trim25); leukocyte cell-derived chemotaxin 2 precursor (lect2).

ISSN: 1471-2164