Identification of common carp (Cyprinus carpio) microRNAs and microRNA-related SNPs

BMC Genomics

Table 2 The effect of SNPs on miRNA precursors energy change

Precursor	SNP position in precursor	SNP	SNP location	△△G (kcal/mol)
s0008	19	A → C	mature	−0.3
s0027-1	53	U → G	mature	−1.4
s0008	39	C → G	Stem	2.2
s0009	41	U → C	Stem	−0.5
s0015	48	C → A	Stem	0.3
s0015	50	U → C	Stem	−1.3
s0027-1	52	G → U	Stem	0.6
s0007	43	G → A	Stem	−0.1
mir-140	43	G → A	Loop	0
s0007	39	A → C	Loop	0
s0009	45	U → G	Loop	0
s0013	40	C → U	anti-stem	2.7
s0013	41	A → G	anti-stem	1

MFE: minimum free energy.
ΔΔG: the MFE difference value between SNP-type precursors and wild-type precursors. The minus value indicated that the SNP-type precursors had lower structure energy than the wild precursors. Otherwise, the former had higher structure energy than the latter.

ISSN: 1471-2164