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Figure 2 | BMC Genomics

Figure 2

From: Improving mapping and SNP-calling performance in multiplexed targeted next-generation sequencing

Figure 2

Mapping improvements using different bioinformatics tools and settings for the same raw sequencing data (third stage of our multiplexed t NGS test model). This figure illustrates the mapping performance of different tools for the human BRCA1 and BRCA2 sequence reads. From Corona Lite’s default mapping (4 mismatches in 50 colors) to SAET 2.2 read enhancement and subsequent Bioscope 1.0.1 mapping, the number of uniquely mappable reads increased by about 60%. SAET alone improved mappable reads by about 15%-20% and Bioscope improved mappable reads by about 35%-75%. Bioscope 1.0.1 improves coverage, because it can map a sub-segment of a read. As Corona Lite mapped less reads to the target for the 20-plex spot and the non-barcoded Chinese control spot, Bioscope’s segment-mapping approach over-proportionally increased mapped reads by a factor of 2.13 and 1.74.

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