Transcriptomic and metabolomic profiling of chicken adipose tissue in response to insulin neutralization and fasting

Table 1 Fold change verification of gene expression by RT-PCR

Gene symbol	Gene title	Microarray fold change			Quantitative PCR fold change
Gene symbol	Gene title	Fasting vs. Fed	Ins-Neu vs. Fed	Ins-Neu vs. Fasting	Fasting vs. Fed	Ins-Neu vs. Fed	Ins-Neu vs. Fasting
FBXO8	F-box protein 8	4.16***	2.09**	−1.99***	2.59***	2.76***	1.06
DUSP5	Dual specificity phosphatase 5	9.43***	1.05	−8.98***	8.13***	1.69***	−4.82***
BNIP3	BCL2/adenovirus E1B 19 kDa interacting protein 3	2.98***	1.53	−1.95**	3.45***	2.26***	−1.52*
PBX3	Pre-B-cell leukemia homeobox 3	−1.62***	1.07	1.75***	−1.22	1.83***	2.22***
IL10RB	Interleukin 10 receptor, beta	1.74***	1.02	−1.71**	1.76***	1.42*	−1.24
EGR1	Early growth response 1	2.43*	−1.58	−3.86**	2.58***	−1.36	−3.52***
NAB1	NGFI-A binding protein 1 (EGR1 binding protein 1)	2.43***	1.06	−2.29***	1.67**	1.52**	−1.10
PDK4	Pyruvate dehydrogenase kinase, isozyme 4	17.28***	7.06**	−2.45**	18.33***	4.01***	−4.57***
CTSL2	Cathepsin L2	2.09***	1.55*	−1.35*	2.96***	2.053***	−1.44
AGTR1	Angiotensin II receptor, type 1	4.15 ***	2.05*	−2.02**	3.52**	1.72*	−2.05*
SESN1	Sestrin 1	1.86**	1.125	−1.65	1.58*	1.47*	1.07

ISSN: 1471-2164