Figure 4

miR-31 is involved in EPC activities. ( A-C) Knocking down endogenous miR-31 in CB-EPCs. Anti-miR-31 antagomiR or the siGFP control (Ctrl) was introduced into CB-EPCs by electroporation, and 2 days later EPCs were subjected to tube formation ( A) and Transwell cell migration assays ( B). Cellular miR-31 levels were detected by RT-qPCR ( C, left panel; n = 3), and migration assay and tube formation assay results were also quantified ( C, middle and right panels; n = 3, using cells from 3 batches of donors). ( D) Overexpressing miR-31 in PB-EPCs stimulates EPC angiogenic abilities. Cellular miR-31 levels were detected by RT-qPCR (left panel; n = 3). Cellular migration assays and tube formation assays were done, and results were quantified (middle and right panels; n = 3, using cells from 3 batches of donors).