Effects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar)

Table 5 Proteins differentially regulated by diet

Spot No	Protein ID	Accession No. (NCBInr)	Theoretical Mw (KDa)/pI	Protein Score^a	No. Matched peptides (MS/MS)^b	Best score peptide	VO/FO Lean	VO/FO Fat	p-value
1136	Epoxide hydrolase 2 (EPHX2)	gb\|ACI33129.1\|	54.2/5.52	84	1	GGLFVGLPDEIPR	1.23	1.40	0.0190
1148	Hemopexin-like protein* (HPX)	emb\|CAA92147.1\|	50.4/5.61	169	1	VHLDAITSDDAGNIYAFR	1.32	1.56	0.0002
1151	Hemopexin-like protein* (HPX)	ref\|NP_001104617.1\|	51.0/6.18	161	1	VHLDAITSDDAGNIYAFR	1.38	1.54	0.0001
2683	Peroxiredoxin 1 (PRDX1)	gb\|ACI67145.1\|	22.0/6.42	757	8	SISTDYGVLKEDEGIAYR	1.14	1.09	0.0310
3168	2-peptidylprolyl isomerase A (PPIA)	ref\|NP_001135161.1\|	17.5/7.61	101	1	VYFDITIGDTPAGR	−1.46	−1.18	0.0170
3243	Myosin light chain smooth muscle isoform (MYL)	ref\|NP_998803.1\|	16.9/4.47	100	2	EAFLLFDR	−1.23	−1.11	0.0490
3331	similar to H2A histone family, member J (H2A)	ref\|XP_001521566.1\|	13.3/10.84	74	1	AGLQFPVGR	−1.58	−1.18	0.0250
3334	Histone H2A** (H2A)	emb\|CAA25528.1\|	13.7/10.88	37	1	AGLQFPVGR	−1.48	−1.18	0.0210
3445	Galectin 2 (LGALS2)	gb\|ACN10131.1\|	14.8/5.93	314	2	SGASSFSINIGHDSDNYALHFNPR	1.17	1.15	0.0170

^a The protein score probability limit (where P < 0.05) is 73.
^b Peptides with confidence interval above 95% were considered.
* Equivalent to warm-temperature-acclimation-related-65 kDa-protein.
**Same protein identification obtained by MS/PMF (peptide mass fingerprinting): Score = 76; 7 peptides matched.
Only reliable identifications of Actinopterygii, obtained by mass spectrometry (MALDI-TOF-MS/MS) analysis and searches in MASCOT, NCBI and ExPASy (Mw/pI) databases are shown. Expression ratios between fish fed VO and FO diets are given for each family group, as well as p-value for diet (two-way ANOVA, DeCyder V7.0).

ISSN: 1471-2164