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Table 6 Proteins differentially regulated by genotype

From: Effects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar)

Spot No

Protein ID

Accession No. (NCBInr)

Theoretical Mw (KDa)/pI

Protein Scorea

No. Matched peptides (MS/MS)b

Best score peptide

Lean/Fat FO

Lean/Fat VO

p-value

1148

Hemopexin-like protein* (HPX)

emb|CAA92147.1|

50.4/5.61

169

1

VHLDAITSDDAGNIYAFR

−1.09

−1.29

0.0270

1389

Calreticulin precursor (CALR)

gb|ACI32936.1|

47.6/4.33

81

2

FEPFSNEGK

−1.28

−1.33

0.0026

1441

Alpha-enolase (ENO1)

ref|NP_001133366.1|

47.0/5.91

405

3

AAVPSGASTGIYEALELR

1.28

1.2

0.0066

1714

Heat shock protein 70 (HSP70)

ref|NP_990334.1|

70.8/5.47

111

1

IINEPTAAAIAYGLDKK

1.16

1.77

0.0260

2134

Heterogeneous nuclear ribonucleoprotein A0 (HNRNPA0)

gb|ACI67551.1|

28.8/9.10

235

2

LFVGGLNVDTDDDGLRK

−1.19

−1.09

0.0460

2154

Calponin 1 (CNN1)

ref|NP_001139857.1|

33.2/8.56

565

4

KINTSPQNWHQLENIGNFVR

−1.34

−1.51

0.0350

2157

Calponin 1*** (CNN1)

ref|NP_001139857.1|

33.2/8.56

43

1

YDPQKEEELR

−1.34

−1.47

0.0240

2213

Caspase 3 (CASP3)

ref|NP_001133393.1|

31.0/5.97

254

2

IPVEADFLYAYSTAPGYYSWR

−1.15

−1.24

0.0300

2215

Caspase 3 (CASP3)

ref|NP_001133393.1|

31.0/5.97

279

2

VANDQTVQQIQQLLSK

−1.14

−1.31

0.0130

2282

Voltage-dependent anion channel 2–2 (VDAC2)

gb|ACH71030.1|

30.1/8.85

214

1

VNNNSLVGVGYTQTLRPGVK

−1.24

−1.19

0.0160

2458

Annexin A4 (ANXA4)

gb|ACI69495.1|

28.4/5.22

218

3

NHLLQVFK

−1.14

−1.11

0.0300

2487

Dihydropteridine reductase (DHPR)

gb|ACI67281.1|

15.7/8.46

81

1

QSVWTSTISSHLATR

−1.14

−1.11

0.0300

2499

Pancreatic alpha-amylase precursor (AMY2)

ref|NP_001036176.1|

57.4/6.89

86

1

ALVFVDNHDNQR

−1.37

−1.27

0.0021

2522

Triosephosphate isomerase 1b (TPI1)

ref|NP_001133174.1|

26.6/7.63

336

2

LDPNTEVVCGAPSIYLEFAR

1.26

1.16

0.0009

2527

Histone cluster 1 (H2A)

ref|NP_001086775.1|

14.0/10.88

94

1

AGLQFPVGR

1.12

1.11

0.0074

2677

Transgelin (TAGLN)

gb|ACM09025.1|

21.7/7.69

219

1

DGCVLSELINSLHK

−1.61

−1.35

0.0350

2717

Proteasome beta 1 subunit (PSMB1)

ref|NP_001003889.1|

26.1/6.32

87

1

GAVYSFDPVGSYQR

−1.29

−1.12

0.0140

2903

Peroxiredoxin-1(PRDX1)

gb|ACI67145.1|

22.0/6.42

115

1

QITINDLPVGR

−1.62

−1.99

0.0069

3345

Retinol-binding protein II, cellular (RBP2)

ref|NP_001139954.1|

15.6/5.44

70

2

AIDIDFATR

−1.32

−1.1

0.0330

3457

Alpha globin (HBA)

emb|CAA65949.1|

15.1/9.19

170

2

TYFSHWADLSPGSAPVK

1.03

2.09

0.0320

  1. aThe protein score probability limit (where P < 0.05) is 73.
  2. b Peptides with confidence interval above 95% were considered.
  3. * Equivalent to warm-temperature-acclimation-related-65 kDa-protein.
  4. *** Same protein identification obtained by MS/PMF (peptide mass fingerprinting): Score = 193; 27 peptides matched.
  5. Only reliable identifications of Actinopterygii, obtained by mass spectrometry (MALDI-TOF-MS/MS) analysis and searches in MASCOT, NCBI and ExPASy (Mw/pI) databases are shown. Expression ratios between Lean and Fat family groups are given for each dietary treatment, as well as p-value for genotype (two-way ANOVA, DeCyder V7.0).